Interaction of a rhizobial DNA-binding protein with the promoter region of a plant leghemoglobin gene
- Max Planck Insitut fur Zuchtungsforschung, Koeln (Germany)
- Michigan State Univ., East Lansing, MI (United States)
- Max Planck Institut fur Zuchtungsforschung, Koeln (Germany) Michigan State Univ., East Lansing, MI (United States)
A nucleotide sequence was identified approximately 650 bp upstream of the Sesbania rostrata leghemoglobin gene Srglb3 start codon, which interacts specifically with a proteinaceous DNA-binding factor found in nodule extracts but not in extracts from leaves or root. The binding site for this factor was delimited using footprinting techniques. The DNA-binding activity of this factor was found to be heat stable, dependent on divalent cations, and derived from the (infecting) Azorhizobium caulinodans bacteria or bacteroids (A. caulinodans bacterial binding factor 1, AcBBF1). A 9- to 10-kD protein was isolated from a free-living culture of A. caulinodans that co-purifies with the DNA-binding activity (A. caulinodans bacterial binding protein 1, AcBBP1) and interacts specifically with its target (S. rostrata bacterial binding site 1, SrBBS1). The amino acid sequence of the N-terminal 27 residues of AcBBP1 was determined and was found to share significant similarity (46% identity; 68% similarity) with a domain of the herpes simplex virus major DNA-binding protein infected cell protein 8(ICP8). An insertion mutation in the SrBBS1 was found to result in a substantial reduction of the expression of a Srglb3-gus reporter gene fusion in nodules of transgenic Lotus corniculatus plants, suggesting a role for this element in Srglb3 promoter activity. Based on these results, the authors propose that (a) bacterial transacting factor(s) may play a role in infected cell-specific expression of the symbiotically induced plant lb genes. 70 refs., 11 figs.
- OSTI ID:
- 5814999
- Journal Information:
- Plant Physiology; (United States), Vol. 102:4; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LEGUMINOSAE
GENES
PROTEINS
AMINO ACID SEQUENCE
DNA
DNA SEQUENCING
NUCLEOTIDES
PLANTS
RHIZOBIUM
ROOTS
BACTERIA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry
550400 - Genetics