Role of the RS1 sequence of the cholera vibrio in amplification of the segment of plasmid DNA carrying the gene of resistance to tetracycline and the genes of cholera toxin

Journal Article · Tue Nov 01 00:00:00 EST 1988 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5814937

Fil'kova, S L; Il'ina, T S; Gintsburg, A L; Yanishevskii, N V; Smirnov, G B

The hybrid plasmid pCO107, representing cointegrate 14(2)-5(2) of two plasmids, an F-derivative (pOX38) and a PBR322-derivative (pCT105) with an RS1 sequence of the cholera vibrio cloned in its makeup, contains two copes of RS1 at the sites of union of the two plasmids. Using a tetracycline resistance marker (Tc/sup R/) of the plasmid pCT105, clones were isolated which have an elevated level of resistance to tetracycline (an increase of from 4- to 30-fold). Using restriction analysis and the Southern blot method of hybridization it was shown that the increase in the level of resistance of tetracycline is associated with the amplification of pCT105 portion of the cointegrate, and that the process of amplification is governed by the presence of direct repeats of the RS1 sequence at its ends. The increase in the number of copies of the pCT105 segment, which contains in its composition the genes of cholera toxin (vct), is accompanied by an increase in toxin production.

• Research Organization: N.F. Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Moscow (USSR)
• OSTI ID: 5814937
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Vol. 24:5; Other Information: Translated from Genetika; 24: No. 5, 821-828(May 1988)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDONUCLEASES
BIOLOGICAL EFFECTS
GENE AMPLIFICATION
TOXINS
BIOSYNTHESIS
CHOLERA
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ESCHERICHIA COLI
GENETIC MAPPING
HYBRIDIZATION
LABELLED COMPOUNDS
MOLECULAR BIOLOGY
PLASMIDS
RADIOENZYMATIC ASSAY
TETRACYCLINES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIGENS
BACTERIA
BACTERIAL DISEASES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DISEASES
DNA HYBRIDIZATION
DNA-ASE
DRUGS
ENZYMES
ESTERASES
HYDROLASES
HYDROLYSIS
INFECTIOUS DISEASES
LYSIS
MAPPING
MATERIALS
MICROORGANISMS
PHOSPHODIESTERASES
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TOXIC MATERIALS
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