Effect of hypophysectomy and hormonal replacement on the uptake of Tc-99m methylene diphosphonate in the metaphysis and shaft of the rat femur: concise communication

Journal Article · Tue Mar 01 00:00:00 EST 1983 · J. Nucl. Med.; (United States)

OSTI ID:5814280

Trow, R S; Klingensmith, G J; Klingensmith, W C; Huffer, W E; Schalch, D S

We have investigated the uptake of Tc-99m methylene diphosphonate (Tc-MDP) in the metaphysis and shaft of the rat femur as affected by hypophysectomy and hormonal replacement with growth hormone and thyroxine. Two hours following injection of Tc-MDP, the metaphysis and a specimen of shaft were obtained and the metaphysis-to-shaft radioactivity ratio was measured. By five days after hypophysectomy the metaphysis-to-shaft ratio fell from a control value of 3.8 +/- 0.2 (mean +/- s.e.) to 2.4 +/- 0.2 (p less than 0.05) and remained significantly decreased throughout the 30-day study. When daily hormonal replacement with 0.5 mg of bovine growth hormone and 10 micrograms of thyroxine (both administered intraperitoneally) was given, beginning on the eighth day after hypophysectomy, the metaphysis-to-shaft ratio of Tc-MDP returned to control levels in twelve days. This model demonstrates the effect of growth hormone and thyroxine on the distribution of Tc-MDP, and may be useful as a radiobioassay of net circulating skeletal growth-promoting activity.

• Research Organization: Veterans Administration Medical Center, Denver, Colorado
• OSTI ID: 5814280
• Journal Information: J. Nucl. Med.; (United States), Vol. 24:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FEMUR
RADIONUCLIDE KINETICS
HYPOPHYSECTOMY
BIOLOGICAL EFFECTS
STH
TECHNETIUM 99
UPTAKE
THYROXINE
ISOMERIC NUCLEI
RATS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
HORMONES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
RODENTS
SKELETON
SURGERY
TECHNETIUM ISOTOPES
THYROID HORMONES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)