A comparison of the effects of two methods of acclimation of aerobic biodegradability
- Eastman Kodak Co., Rochester, NJ (United States). Environmental Sciences Section
The acclimation or adaptation of microorganisms to organic chemicals is an important factor influencing both the rate and the extent of biodegradation. In this study two acclimation procedures were evaluated in terms of their effectiveness in enhancing biodegradation, their relative ease of use in the laboratory, and the implications for biodegradability testing. In the single-flask procedure, microorganisms were acclimated for 2 to 7 d in a single acclimation flask at constant or increasing concentrations of the test chemical without transfer of microorganisms. In the second procedure, the enrichment procedure, microorganisms were acclimated in a series of flasks over a 21-d period by making adaptive transfers to increasing concentrations of the test chemical. Acclimated microorganisms from each procedure were used as the source of inoculum for subsequent biodegradation tests in which carbon dioxide evolution was measured. Six chemicals were tested: quinoline, p-nitrophenol, N-methylaniline, N,N-dimethylaniline, acrylonitrile, and 2,2,4-trimethyl-1,3-pentanediol monoisobutyrate. Microorganisms acclimated in the single-flask procedure were much more effective than those acclimated in the enrichment procedure in degrading the test chemicals. The single-flask procedure is more convenient to use, and it permits monitoring of the time needed for acclimation. The results from these studies have implications for the methodology used in biodegradation test systems and suggest caution before adopting a multiple-flask, enrichment acclimation procedure before the performance of standardized tests for aerobic biodegradability.
- OSTI ID:
- 5810253
- Journal Information:
- Environmental Toxicology and Chemistry; (United States), Vol. 12:11; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACRYLONITRILE
BIODEGRADATION
ANILINE
ISOBUTYRIC ACID
MICROORGANISMS
BIOLOGICAL ADAPTATION
NITROPHENOL
QUINOLINES
AEROBIC CONDITIONS
COMPARATIVE EVALUATIONS
AMINES
AROMATICS
AZAARENES
AZINES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
EVALUATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MONOCARBOXYLIC ACIDS
NITRILES
NITRO COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
PYRIDINES
560300* - Chemicals Metabolism & Toxicology