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Title: Iodine 125-lysergic acid diethylamide binds to a novel serotonergic site on rat choroid plexus epithelial cells

Abstract

/sup 125/I-Lysergic acid diethylamide (/sup 125/I-LSD) binds with high affinity to serotonergic sites on rat choroid plexus. These sites were localized to choroid plexus epithelial cells by use of a novel high resolution stripping film technique for light microscopic autoradiography. In membrane preparations from rat choroid plexus, the serotonergic site density was 3100 fmol/mg of protein, which is 10-fold higher than the density of any other serotonergic site in brain homogenates. The choroid plexus site exhibits a novel pharmacology that does not match the properties of 5-hydroxytryptamine-1a (5-HT1a), 5-HT1b, or 5-HT2 serotonergic sites. /sup 125/I-LSD binding to the choroid plexus site is potently inhibited by mianserin, serotonin, and (+)-LSD. Other serotonergic, dopaminergic, and adrenergic agonists and antagonists exhibit moderate to weak affinities for this site. The rat choroid plexus /sup 125/I-LSD binding site appears to represent a new type of serotonergic site which is located on non-neuronal cells in this tissue.

Authors:
;
Publication Date:
Research Org.:
Johns Hopkins Univ., Baltimore, MD
OSTI Identifier:
5809888
Resource Type:
Journal Article
Journal Name:
J. Neurosci.; (United States)
Additional Journal Information:
Journal Volume: 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; AUTORADIOGRAPHY; SEROTONIN; RECEPTORS; AMIDES; CATTLE; CHEMICAL BONDS; EPITHELIUM; IODINE 125; LABELLED COMPOUNDS; LYSERGIC ACID; MICE; RABBITS; RATS; SWINE; TRACER TECHNIQUES; ALKALOIDS; AMINES; ANIMAL TISSUES; ANIMALS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZAARENES; AZOLES; BETA DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PROTEINS; PYRROLES; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; RODENTS; RUMINANTS; SYMPATHOMIMETICS; TISSUES; TRYPTAMINES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Yagaloff, K A, and Hartig, P R. Iodine 125-lysergic acid diethylamide binds to a novel serotonergic site on rat choroid plexus epithelial cells. United States: N. p., 1985. Web.
Yagaloff, K A, & Hartig, P R. Iodine 125-lysergic acid diethylamide binds to a novel serotonergic site on rat choroid plexus epithelial cells. United States.
Yagaloff, K A, and Hartig, P R. 1985. "Iodine 125-lysergic acid diethylamide binds to a novel serotonergic site on rat choroid plexus epithelial cells". United States.
@article{osti_5809888,
title = {Iodine 125-lysergic acid diethylamide binds to a novel serotonergic site on rat choroid plexus epithelial cells},
author = {Yagaloff, K A and Hartig, P R},
abstractNote = {/sup 125/I-Lysergic acid diethylamide (/sup 125/I-LSD) binds with high affinity to serotonergic sites on rat choroid plexus. These sites were localized to choroid plexus epithelial cells by use of a novel high resolution stripping film technique for light microscopic autoradiography. In membrane preparations from rat choroid plexus, the serotonergic site density was 3100 fmol/mg of protein, which is 10-fold higher than the density of any other serotonergic site in brain homogenates. The choroid plexus site exhibits a novel pharmacology that does not match the properties of 5-hydroxytryptamine-1a (5-HT1a), 5-HT1b, or 5-HT2 serotonergic sites. /sup 125/I-LSD binding to the choroid plexus site is potently inhibited by mianserin, serotonin, and (+)-LSD. Other serotonergic, dopaminergic, and adrenergic agonists and antagonists exhibit moderate to weak affinities for this site. The rat choroid plexus /sup 125/I-LSD binding site appears to represent a new type of serotonergic site which is located on non-neuronal cells in this tissue.},
doi = {},
url = {https://www.osti.gov/biblio/5809888}, journal = {J. Neurosci.; (United States)},
number = ,
volume = 12,
place = {United States},
year = {Sun Dec 01 00:00:00 EST 1985},
month = {Sun Dec 01 00:00:00 EST 1985}
}