Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome

Shoemaker, N B; Getty, C; Gardner, J F; Salyers, A A

Title: Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome

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Abstract

The gene for resistance to erythromycin and clindamycin, which is carried on the conjugative Bacteroides plasmid, pBF4, has been shown previously to be part of an element (Tn4351) that transposes in Escherichia coli. The authors have now introduced Tn4351 into Bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range IncP plasmid R751 (R751::Tn4351) and (ii) pSS-2, a chimeric plasmid which contains 33 kilobases of pBF4 (including Tn4351) cloned into the IncQ plasmid RSF1010 and which is mobilized by R751. When E. coli HB101, carrying either R751::Tn4351 or R751 and pSS-2, was mated with B. uniformis under aerobic conditions, Em/sup r/ transconjugants were detected at a frequency of 10 /sup -6/ to 10/sup -5/ (R751::Tn4351) or 10/sup -8/ to 10/sup -6/ (R751 and pSS-2). In matings involving pSS-2, all Em/sup r/ transconjugants contained simple insertions of Tn4351 in the chromosome, whereas in matings involving R751::Tn4351, about half of the Em/sup r/ transconjugants had R751 cointegrated with Tn4351 in the chromosome. Of the Em/sup r/ transconjugants, 13% were auxotrophs. Bacteroides spp. which had R751 cointegrated with Tn4351 in the chromosome did not transfer R751 or Tn4351 to E. coli HB101 or to isogenic B. uniformis, nor did the integratedmore »« less

Authors:: Shoemaker, N B; Getty, C; Gardner, J F; Salyers, A A

Publication Date:: Sat Mar 01 00:00:00 EST 1986

Research Org.:: Univ. of Illinois, Urbana

OSTI Identifier:: 5809462

DOE Contract Number:: AC02-81ER10944

Resource Type:: Journal Article

Journal Name:: J. Bacteriol.; (United States)

Additional Journal Information:: Journal Volume: 165:3

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; PLASMIDS; GENE RECOMBINATION; BACTERIA; CHROMOSOMES; ESCHERICHIA COLI; GENES; GENETICS; BIOLOGY; CELL CONSTITUENTS; MICROORGANISMS; 550400* - Genetics

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                    Shoemaker, N B, Getty, C, Gardner, J F, and Salyers, A A. Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome.  United States: N. p., 1986. 
        Web.

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                    Shoemaker, N B, Getty, C, Gardner, J F, & Salyers, A A. Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome.  United States.

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                    Shoemaker, N B, Getty, C, Gardner, J F, and Salyers, A A. 1986.  
        "Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome".  United States.

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@article{osti_5809462,

  title        = {Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome},

  author       = {Shoemaker, N B and Getty, C and Gardner, J F and Salyers, A A},

  abstractNote = {The gene for resistance to erythromycin and clindamycin, which is carried on the conjugative Bacteroides plasmid, pBF4, has been shown previously to be part of an element (Tn4351) that transposes in Escherichia coli. The authors have now introduced Tn4351 into Bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range IncP plasmid R751 (R751::Tn4351) and (ii) pSS-2, a chimeric plasmid which contains 33 kilobases of pBF4 (including Tn4351) cloned into the IncQ plasmid RSF1010 and which is mobilized by R751. When E. coli HB101, carrying either R751::Tn4351 or R751 and pSS-2, was mated with B. uniformis under aerobic conditions, Em/sup r/ transconjugants were detected at a frequency of 10 /sup -6/ to 10/sup -5/ (R751::Tn4351) or 10/sup -8/ to 10/sup -6/ (R751 and pSS-2). In matings involving pSS-2, all Em/sup r/ transconjugants contained simple insertions of Tn4351 in the chromosome, whereas in matings involving R751::Tn4351, about half of the Em/sup r/ transconjugants had R751 cointegrated with Tn4351 in the chromosome. Of the Em/sup r/ transconjugants, 13% were auxotrophs. Bacteroides spp. which had R751 cointegrated with Tn4351 in the chromosome did not transfer R751 or Tn4351 to E. coli HB101 or to isogenic B. uniformis, nor did the integrated R751 mobilize pE5-2, an E. coli-Bacteroides shuttle vector that contains a transfer origin that is recognized by R751.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5809462},
  journal      = {J. Bacteriol.; (United States)},
number       = ,

  volume       = 165:3,

  place        = {United States},

  year         = {Sat Mar 01 00:00:00 EST 1986},

  month        = {Sat Mar 01 00:00:00 EST 1986}

}

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