Effect of hemopoietic microenvironment on splenic suppressor macrophages in congenitally anemic mice of genotype S1/sup d/
Mechanisms underlying mononuclear phagocyte specialization are being probed by studying suppressor macrophages as a reference population in mouse models with impaired blood monocyte formation. Splenic suppressor macrophages, defined by PGE-mediated inhibition of Con A-induced T lymphocyte proliferation are induced by the i.p. administration of Corynebacterium parvum (CP). Mice severely depleted of bone marrow and blood monocytes by treatment with /sup 89/Sr fail to show this suppressor macrophages response to CP, although macrophages-forming stem cells, assessed as splenic M-CFC in vitro, are increased 20-fold. These observations suggest that radiosensitive bone marrow stem cells are necessary for the generation of both suppressor macrophages and monocytes and that one such stem cell may be common to both types of mononuclear phagocytes. This notion was explored further by employing congenitally anemic mice of the genotype S1/S1 superscript d in which the hemopoietic microenvironment is genetically defective and thus unable to support the proliferation, differentiation, and function of stem cells. The congenital defect was found to be additionally expressed in the S1/S1/sup d/ mouse by a monocytopenia of less than 10% of the values in normal congenic littermate controls and by the failure of splenic M-CFC to increase in response to cp. PGE-producing suppressor macrophages expressing Fcy2b receptors, however, were induced by CP in S1/S1/sup d/ mice with no significant diminution of suppressor activity.
- Research Organization:
- Medical Coll. of Pennsylvania, Philadelphia (USA). Dept. of Microbiology and Immunology
- OSTI ID:
- 5803971
- Report Number(s):
- AD-A-165525/7/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANEMIAS
BLOOD FORMATION
BIOLOGICAL RADIATION EFFECTS
HEMATOPOIETIC SYSTEM
MACROPHAGES
MONOCYTES
STRONTIUM 89
BONE MARROW
GENETICS
GLOBULINS
IMMUNOLOGY
INHIBITION
LYMPHOCYTES
MICE
PHAGOCYTES
SPLEEN
STRONTIUM
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELEMENTS
EVEN-ODD NUCLEI
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
METALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STRONTIUM ISOTOPES
SYMPTOMS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals