Sulfoxide stimulation of chondrogenesis in limb mesenchyme is accompanied by an increase in type II collagen enhancer activity

Journal Article · Wed May 01 00:00:00 EDT 1991 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(91)90136-I· OSTI ID:5803373

Horton, Jr, W E; Higginbotham, J D ^[1]

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1. National Institute on Aging, Baltimore, MD (USA)

We have utilized a modification of the limb bud mesenchyme micromass culture system to screen compounds that might stimulate chondrogenesis. Two compounds in the sulfoxide family (methylphenylsulfoxide and p-chlorophenyl methyl sulfoxide) were stimulatory at 10(-2) M and 10(-3) M, respectively; whereas other sulfoxides and organic solvents were not active at these concentrations. In addition, specific growth factors (basic FGF, IGF-I, IGF-II) were not chondroinductive at concentrations that are active in other cell systems. Both sulfoxide compounds stimulated cartilage nodule formation, ({sup 35}S)sulfate incorporation, and activity of the regulatory sequences of the collagen II gene. In contrast, transforming growth factor beta-1 (10 ng/ml) stimulated sulfate incorporation but produced only a diffuse deposition of cartilage matrix and reduced the ability of the cells to utilize the regulatory sequences of the collagen II gene. The sulfoxides appear to promote the differentiation of limb bud cells to chondrocytes and thus exhibit chondroinductive activity.

• OSTI ID: 5803373
• Journal Information: Experimental Cell Research; (USA), Vol. 194:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARTILAGE
CELL DIFFERENTIATION
COLLAGEN
GENES
SULFOXIDES
BIOCHEMICAL REACTION KINETICS
CHICKENS
FLUORESCENCE
GENE REGULATION
GROWTH FACTORS
ONTOGENESIS
SULFATES
SULFUR 35
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
BODY
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FOWL
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LUMINESCENCE
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SCLEROPROTEINS
SULFUR COMPOUNDS
SULFUR ISOTOPES
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques