Selectively eliminated blood monocytes and splenic suppressor macrophages in mice depleted of bone marrow by strontium 89

Technical Report · Tue Jan 01 00:00:00 EST 1985

OSTI ID:5802744

Dempsey, W L; Morahan, P S

The contribution of specific activity to the effects of the bone-seeking isotope, /sup 89/Sr on radiosensitive components of mononuclear phagocyte populations was investigated in mice. CBA/J mice received a fixed dose of 2 microcuries/g body weight of /sup 89/Sr with three different specific activities, 6 Ci, 100 microcuries, and 20 /sup 89/Sr microcuries per mg Sr. The estimated radioactivity located in the bone surface was 4200, 3000 and 2400 cpm/mg bone when measured 2 days after the administration of /sup 89/Sr, and was lost with an estimated biological half-life of 27, 25, and 23 days, respectively. Bone marrow suppression was assessed by quantitation of the depletion of macrophage-colony forming cells (M-CFC) grown in vitro. The decline in M-CFC closely paralleled the level of radioactivity in the bone. These effects were clearly reflected by the depletion of monocytes in the blood, which were reduced to 14%, 14%, and 21% of control levels corresponding to SA's of 6 Curies/mg, 100 microcuries/mg and 20 microcuries/mg when counted on day 10. By day 30 the respective monocyte levels were 15%, 31%, and 77%. Furthermore, the induction of prostaglandin E producing suppressor macrophages (marophages) by Corynebacterium parvum administration was found to vary inversely with the effects of radioactivity in the bone, with initial impairment followed by quantitative recorvery. Resident-type macrophages in impairment cavity appear to be unaffected by /sup 89/Sr-treament. These data suggest that the monocytes and suppressor macrophages are dependent on radiosensitive marrow cells.

• Research Organization: Medical Coll. of Pennsylvania, Philadelphia (USA). Dept. of Microbiology and Immunology
• OSTI ID: 5802744
• Report Number(s): AD-A-165530/7/XAB
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
BLOOD FORMATION
BIOLOGICAL RADIATION EFFECTS
HEMATOPOIETIC SYSTEM
MACROPHAGES
MONOCYTES
PHAGOCYTES
RADIOSENSITIVITY
STRONTIUM 89
BONE MARROW
MICE
PROSTAGLANDINS
SPLEEN
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEI
ORGANS
RADIATION EFFECTS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STRONTIUM ISOTOPES
SYMPTOMS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals