Mutation and structure-function relationships of cytochrome c
The yeast cytochrome c system has become a key vehicle for structure- function studies in vitro using modern molecular genetic techniques to clarify fundamental aspects of the molecular evolutionary design of iso-1-cytochrome c (cyt c). The spectral properties of cyt c allow estimation of the number of molecules in vivo, and growth in lactate medium allows estimation of cyt c activity. Because most of our studies involve single copy replacements of CYC1, the cyt c gene, specific activities of altered forms of cyt c in vivo can be related to properties determined in vitro. We have identified five classes of cyt c mutants, and suggest mechanisms to account for each class of mutant. Lysine 77 is evolutionarily conserved in most eukaryotes; effect either in vitro or in vivo. CYC7 encodes iso-2-cytochrome c, another form of cyt c. CYC7 contains a non-AUG transcriptional start site, and was used to study initiation of protein synthesis at non- AUG codons. 3 refs.
- Research Organization:
- Rochester Univ., NY (USA). School of Medicine and Dentistry
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG02-88ER60630
- OSTI ID:
- 5802642
- Report Number(s):
- DOE/ER/60630-T1; ON: DE91013320
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CYTOCHROMES
ENZYME ACTIVITY
MUTAGENESIS
STRUCTURE-ACTIVITY RELATIONSHIPS
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
CODONS
GENE MUTATIONS
GENE REPRESSORS
GENETIC ENGINEERING
IN VITRO
IN VIVO
LYSINE
MOLECULAR BIOLOGY
OLIGONUCLEOTIDES
PROGRESS REPORT
RECOMBINANT DNA
SACCHAROMYCES CEREVISIAE
TRANSCRIPTION
TRANSCRIPTION FACTORS
AMINO ACIDS
CARBOXYLIC ACIDS
DNA
DOCUMENT TYPES
EUMYCOTA
FUNGI
MICROORGANISMS
MOLECULAR STRUCTURE
MUTATIONS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
PLANTS
PROTEINS
SACCHAROMYCES
YEASTS
550200* - Biochemistry
550400 - Genetics