Formation of tissue factor activity following incubation of recombinant human tissue factor apoprotein with plasma lipoproteins
- Univ. of New Mexico School of Medicine, Albuquerque (USA)
Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low density and high density lipoproteins resulted in tissue factor activity in a clotting assay. The order of effectiveness was VLDL greater than LDL much greater than HDL. Tissue factor activity generated by incubation of a fixed amount of Apo-TF with plasma lipoproteins was lipoprotein concentration-dependent and saturable. The association of Apo-TF with lipoprotein particles was supported by gel filtration studies in which {sup 125}I-Apo-TF coeluted with the plasma lipoprotein in the void volume of a Superose 6 column in the presence and absence of calcium ions. In addition, void-volume Apo-TF-lipoprotein fractions exhibited tissue factor activity. These results suggest that the factor VIII-bypassing activity of bovine Apo-TF observed in a canine hemophilic model may be due, in part, to its association with plasma lipoproteins and expression of functional tissue factor activity.
- OSTI ID:
- 5802230
- Journal Information:
- Thrombosis Research; (USA), Vol. 60:3; ISSN 0049-3848
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPOPROTEINS
BIOCHEMICAL REACTION KINETICS
THROMBOPLASTIN
BIOSYNTHESIS
DOGS
DOSE-RESPONSE RELATIONSHIPS
IODINE 125
MAN
TIME DEPENDENCE
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HEMATOLOGIC AGENTS
HEMOSTATICS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques