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Title: Formation of tissue factor activity following incubation of recombinant human tissue factor apoprotein with plasma lipoproteins

Abstract

Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low density and high density lipoproteins resulted in tissue factor activity in a clotting assay. The order of effectiveness was VLDL greater than LDL much greater than HDL. Tissue factor activity generated by incubation of a fixed amount of Apo-TF with plasma lipoproteins was lipoprotein concentration-dependent and saturable. The association of Apo-TF with lipoprotein particles was supported by gel filtration studies in which {sup 125}I-Apo-TF coeluted with the plasma lipoprotein in the void volume of a Superose 6 column in the presence and absence of calcium ions. In addition, void-volume Apo-TF-lipoprotein fractions exhibited tissue factor activity. These results suggest that the factor VIII-bypassing activity of bovine Apo-TF observed in a canine hemophilic model may be due, in part, to its association with plasma lipoproteins and expression of functional tissue factor activity.

Authors:
;  [1]
  1. Univ. of New Mexico School of Medicine, Albuquerque (USA)
Publication Date:
OSTI Identifier:
5802230
Resource Type:
Journal Article
Journal Name:
Thrombosis Research; (USA)
Additional Journal Information:
Journal Volume: 60:3; Journal ID: ISSN 0049-3848
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LIPOPROTEINS; BIOCHEMICAL REACTION KINETICS; THROMBOPLASTIN; BIOSYNTHESIS; DOGS; DOSE-RESPONSE RELATIONSHIPS; IODINE 125; MAN; TIME DEPENDENCE; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BLOOD COAGULATION FACTORS; COAGULANTS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HEMATOLOGIC AGENTS; HEMOSTATICS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIPIDS; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; SYNTHESIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Sakai, T, and Kisiel, W. Formation of tissue factor activity following incubation of recombinant human tissue factor apoprotein with plasma lipoproteins. United States: N. p., 1990. Web. doi:10.1016/0049-3848(90)90182-C.
Sakai, T, & Kisiel, W. Formation of tissue factor activity following incubation of recombinant human tissue factor apoprotein with plasma lipoproteins. United States. https://doi.org/10.1016/0049-3848(90)90182-C
Sakai, T, and Kisiel, W. 1990. "Formation of tissue factor activity following incubation of recombinant human tissue factor apoprotein with plasma lipoproteins". United States. https://doi.org/10.1016/0049-3848(90)90182-C.
@article{osti_5802230,
title = {Formation of tissue factor activity following incubation of recombinant human tissue factor apoprotein with plasma lipoproteins},
author = {Sakai, T and Kisiel, W},
abstractNote = {Incubation of recombinant human tissue factor apoprotein (Apo-TF) with human plasma decreased the recalcified clotting time of this plasma in a time-and dose-dependent manner suggesting relipidation of the Apo-TF by plasma lipoproteins. Incubation of Apo-TF with purified preparations of human very low density, low density and high density lipoproteins resulted in tissue factor activity in a clotting assay. The order of effectiveness was VLDL greater than LDL much greater than HDL. Tissue factor activity generated by incubation of a fixed amount of Apo-TF with plasma lipoproteins was lipoprotein concentration-dependent and saturable. The association of Apo-TF with lipoprotein particles was supported by gel filtration studies in which {sup 125}I-Apo-TF coeluted with the plasma lipoprotein in the void volume of a Superose 6 column in the presence and absence of calcium ions. In addition, void-volume Apo-TF-lipoprotein fractions exhibited tissue factor activity. These results suggest that the factor VIII-bypassing activity of bovine Apo-TF observed in a canine hemophilic model may be due, in part, to its association with plasma lipoproteins and expression of functional tissue factor activity.},
doi = {10.1016/0049-3848(90)90182-C},
url = {https://www.osti.gov/biblio/5802230}, journal = {Thrombosis Research; (USA)},
issn = {0049-3848},
number = ,
volume = 60:3,
place = {United States},
year = {Thu Nov 01 00:00:00 EST 1990},
month = {Thu Nov 01 00:00:00 EST 1990}
}