Nicotinic cholinergic receptors in rat brain. Annual report No. 3, 1 May 85-30 Apr 86
We have compared the characteristics of the recognition sites for 3(H)acetylcholine and 3H(-)nicotine in rat brain and found that the pharmacology, distribution, disulfide bond requirement, and regulation by chronic administration of nicotine and soman are identical. From these studies we conclude that 3Hacetylcholine and 3H(-)nicotine recognize the same recognition site which has the characteristics expected of a nicotinic cholinergic receptor. We have also determined that 3Hacetylcholine of high specific radioactivity (80 Ci/mmol) is an excellent ligand with which to study muscarinic receptors that have high affinity for agonists. These receptors may represent a subtype of muscarinic receptors found in brain, heart, glands, an some smooth muscle. (JS)
- Research Organization:
- Georgetown Univ., Washington, DC (USA). School of Medicine
- OSTI ID:
- 5801989
- Report Number(s):
- AD-A-230245/3/XAB; CNN: DAMD17-83-C-3113
- Country of Publication:
- United States
- Language:
- English
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ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
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550201* - Biochemistry- Tracer Techniques