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Title: Determination of /sup 35/S-aminoacyl-transfer ribonucleic acid specific radioactivity in small tissue samples

Abstract

Rate determination of protein synthesis utilizing tracer amino acid incorporation requires accurate assessment of the specific radioactivity of the labeled precursor aminoacyl-tRNA pool. Previously published methods presumably useful for the measurement of any aminoacyl-tRNA were unsuccessful when applied to (/sup 35/S)methionine, due to the unique chemical properties of this amino acid. Herein we describe modifications of these methods necessary for the measurement of /sup 35/S-aminoacyl-tRNA specific radioactivity from small tissue samples incubated in the presence of (/sup 35/S)methionine. The use of (/sup 35/S)methionine of high specific radioactivity enables analysis of the methionyl-tRNA from less than 100 mg of tissue. Conditions for optimal recovery of /sup 35/S-labeled dansyl-amino acid derivatives are presented and possible applications of this method are discussed.

Authors:
; ; ;
Publication Date:
Research Org.:
Northwestern Univ. School of Medicine, Chicago, IL
OSTI Identifier:
5801919
Resource Type:
Journal Article
Journal Name:
Anal. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 118:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; METHIONINE; MEASURING METHODS; RADIOASSAY; PROTEINS; BIOSYNTHESIS; SULFUR COMPOUNDS; DOUBLE LABELLING; TRANSFER RNA; TRITIUM COMPOUNDS; BIOCHEMICAL REACTION KINETICS; EXPERIMENTAL DATA; IN VITRO; ISOMERIC NUCLEI; MYOCARDIUM; RABBITS; SULFUR 35; TRACER TECHNIQUES; UPTAKE; AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; DATA; DAYS LIVING RADIOISOTOPES; DRUGS; EVEN-ODD NUCLEI; HEART; INFORMATION; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LABELLED COMPOUNDS; LABELLING; LIGHT NUCLEI; LIPOTROPIC FACTORS; MAMMALS; MUSCLES; NUCLEI; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; RADIOISOTOPES; REACTION KINETICS; RNA; SULFUR ISOTOPES; SYNTHESIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Samarel, A M, Ogunro, E A, Ferguson, A G, and Lesch, M. Determination of /sup 35/S-aminoacyl-transfer ribonucleic acid specific radioactivity in small tissue samples. United States: N. p., 1981. Web. doi:10.1016/0003-2697(81)90172-X.
Samarel, A M, Ogunro, E A, Ferguson, A G, & Lesch, M. Determination of /sup 35/S-aminoacyl-transfer ribonucleic acid specific radioactivity in small tissue samples. United States. https://doi.org/10.1016/0003-2697(81)90172-X
Samarel, A M, Ogunro, E A, Ferguson, A G, and Lesch, M. 1981. "Determination of /sup 35/S-aminoacyl-transfer ribonucleic acid specific radioactivity in small tissue samples". United States. https://doi.org/10.1016/0003-2697(81)90172-X.
@article{osti_5801919,
title = {Determination of /sup 35/S-aminoacyl-transfer ribonucleic acid specific radioactivity in small tissue samples},
author = {Samarel, A M and Ogunro, E A and Ferguson, A G and Lesch, M},
abstractNote = {Rate determination of protein synthesis utilizing tracer amino acid incorporation requires accurate assessment of the specific radioactivity of the labeled precursor aminoacyl-tRNA pool. Previously published methods presumably useful for the measurement of any aminoacyl-tRNA were unsuccessful when applied to (/sup 35/S)methionine, due to the unique chemical properties of this amino acid. Herein we describe modifications of these methods necessary for the measurement of /sup 35/S-aminoacyl-tRNA specific radioactivity from small tissue samples incubated in the presence of (/sup 35/S)methionine. The use of (/sup 35/S)methionine of high specific radioactivity enables analysis of the methionyl-tRNA from less than 100 mg of tissue. Conditions for optimal recovery of /sup 35/S-labeled dansyl-amino acid derivatives are presented and possible applications of this method are discussed.},
doi = {10.1016/0003-2697(81)90172-X},
url = {https://www.osti.gov/biblio/5801919}, journal = {Anal. Biochem.; (United States)},
number = ,
volume = 118:1,
place = {United States},
year = {Sun Nov 15 00:00:00 EST 1981},
month = {Sun Nov 15 00:00:00 EST 1981}
}