Chemical composition and genotoxic activity of petroleum derivatives collected in two working environments

Journal Article · Sun Jan 01 04:00:00 UTC 1989 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398909531293· OSTI ID:5788491

Pasquini, R; Taningher, M; Monarca, S; Pala, M; Angeli, G

Pitch and bitumen, two complex petroleum derivative mixtures, were studied for both their chemical composition and their mutagenic/DNA damaging activity. While bitumen revealed no genotoxic effect and low polycyclic aromatic hydrocarbons (PAHs) concentration, petroleum pitch showed a high concentration of mutagenic/carcinogenic PAHs, and also an elevated mutagenic activity when assayed by the Ames test, in the presence of postmitochondrial rat liver fractions. The in vitro mutagenic activity was detectable as frameshift mutation by assaying the pitch both as an in toto mixture and after HPLC fractionation, the most polar fractions being the most active. In contrast, both derivatives showed no in vivo DNA damage in rat liver, using the DNA alkaline elution technique and the fluorometric assay of DNA unwinding.

• Research Organization: Universita di Perugia (Italy)
• OSTI ID: 5788491
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 27:2; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020600* -- Petroleum-- Health & Safety
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BACTERIA
BODY
CELL CONSTITUENTS
CHEMICAL COMPOSITION
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DNA
ENERGY SOURCES
ENVIRONMENTAL EXPOSURE
FOSSIL FUELS
FRACTIONATION
FUELS
GLANDS
HYDROCARBONS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
METABOLIC ACTIVATION
MICROORGANISMS
MITOCHONDRIA
MUTAGEN SCREENING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PETROLEUM
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES
TOXICITY
VERTEBRATES