Regions of the polytene chromosomes of Drosophila virilis carrying multiple dispersed p Dv 111 DNA sequences
The cloned sequences of p Dv 111 DNA hybridized in situ with more than 170 regions of Drosophila virilis salivary gland chromosomes. Comparative autoradiography of in situ hybridization and the nature of pulse /sup 3/H-thymidine and /sup 3/H-deoxycytidine incorporation into the polytene chromosomes of D. virilis at the puparium formation stage showed that the hybridization sites of p Dv 111 are distributed not only in the heterochromatic regions but also in the euchromatic regions of the chromosomes that are not late replicating. Two distinct bands of hybridization of p Dv 111 /sup 3/H-DNA were observed in the region of the heat shock puff 20CD. The regions of the distal end of chromosome 2, in which breaks appeared during radiation-induced chromosomal rearrangements, hybridized with the p Dv 111 DNA.
- Research Organization:
- Institute of Molecular Biology and Genetics, Kiev, USSR
- OSTI ID:
- 5785043
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 22:3; Other Information: Translated from Genetika; 22: No. 3, 457-466(Mar 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMES
DNA REPLICATION
DNA SEQUENCING
DNA
HYBRIDIZATION
TRACER TECHNIQUES
DROSOPHILA
GENETICS
AUTORADIOGRAPHY
BIOLOGICAL SHOCK
CHROMOSOMAL ABERRATIONS
GENES
LABELLING
PUPAE
RADIONUCLIDE KINETICS
STRAND BREAKS
THERMAL SHOCK
THYMIDINE
TRANSCRIPTION
TRITIUM COMPOUNDS
UPTAKE
ANIMALS
ARTHROPODS
AZINES
BIOLOGY
DIPTERA
FLIES
FRUIT FLIES
HETEROCYCLIC COMPOUNDS
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MUTATIONS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PYRIMIDINES
RIBOSIDES
STRUCTURAL CHEMICAL ANALYSIS
550401* - Genetics- Tracer Techniques