Identification of Schistosoma mansoni glycoproteins recognized by protective antibodies from mice immunized with irradiated cercariae
The humoral immune responses of mice patently infected with Schistosoma mansoni and of mice vaccinated with radiation-attenuated cercariae were compared by radioimmunoassays and one-and two-dimensional polyacrylamide gel analyses of radioimmunoprecipitates. Sera of vaccinated mice precipitated a restricted number of predominantly high m.w. glycoproteins of both schistosomula and adult worms metabolically labeled with (/sup 35/S) methinonine. Each of the glycoproteins of 36 hr in vitro-cultured schistosomula that was precipitated by the sera of vaccinated mice was also precipitated by sera of infected mice. In contrast, sera of vaccinated mice uniquely precipitated a 38,000 m.w. glycoprotein of schistosomula cultured for 5 days and a 94,000 m.w. glycoprotein of adult male worms. Although radiation-attenuated larvae do not reach the adult stage, mice vaccinated with these still elicit a strong immune response against egg glycoproteins. In particular, an egg glycoprotein of 85,000 to 70,000 and isoelectric point of 4.8 showed an enhanced reactivity with sera of vaccinated mice in comparison with infected mice. These results show that the antibody response in mice vaccinated with radiation-attenuated larvae differs qualitatively and quantitatively from that of infected mice.
- Research Organization:
- John Hopkins Univ. School of Medicine, Baltimore, MD
- OSTI ID:
- 5783714
- Journal Information:
- J. Immunol.; (United States), Vol. 136
- Country of Publication:
- United States
- Language:
- English
Similar Records
Schistosoma mansoni polypeptides immunogenic in mice vaccinated with radiation-attenuated cercariae
Identification of surface antigens of schistosomula of Schistosoma mansoni recognized by antibodies from mice immunized by chronic infection and by exposure to highly irradiated cercariae
Related Subjects
SCHISTOSOMA
GLUCOPROTEINS
ANTIBODIES
BIOLOGICAL RADIATION EFFECTS
ELECTROPHORESIS
IMMUNE REACTIONS
LABELLED COMPOUNDS
MICE
PARASITES
RADIOIMMUNOASSAY
RADIORECEPTOR ASSAY
SULFUR 35
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HELMINTHS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC COMPOUNDS
PLATYHELMINTHS
PROTEINS
RADIATION EFFECTS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SACCHARIDES
SULFUR ISOTOPES
TREMATODES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques