Structural organization of replicon domains during DNA synthetic phase in the mammalian nucleus

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(86)90583-5· OSTI ID:5783588

Nakamura, H; Morita, T; Sato, C

In mammalian cells, it has been shown that adjacent multiple DNA replicons, termed a replicon cluster or a replicon domain, are replicated coordinately in a defined temporal order during the DNA synthetic (S) phase. However, no intranuclear structure of this replicon domain has been revealed in the nucleus labelled with (/sup 3/H)thymidine at the limited resolution level of autoradiography. Bu immunofluorescent staining with antibody against 5-bromodeoxyuridine (BrdU), we succeeded in detecting novel, intranuclear ring-like structures of replicating replicon domains that were organized temporarily during the S phase of mammalian cells with incorporated BrdU.

• Research Organization: Aichi Cancer Center Research Institute, Nagoya, Japan
• OSTI ID: 5783588
• Journal Information: Exp. Cell Res.; (United States), Vol. 165
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BUDR
ANTIGEN-ANTIBODY REACTIONS
DNA
BIOSYNTHESIS
AUTORADIOGRAPHY
CELL CULTURES
CELL CYCLE
CELL NUCLEI
LABELLED COMPOUNDS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANTIMETABOLITES
AZINES
BROMOURACILS
CELL CONSTITUENTS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SYNTHESIS
URACILS
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques
550401 - Genetics- Tracer Techniques