Assessment of the TLC/Salmonella assay for screening hazardous wastes
Using a modified version of the TLC/Salmonella assay developed by Bjorseth et al. (1982), 10 complex hazardous wastes were tested for mutagenic activity. The method couples thin-layer chromatography (TLC) with the Salmonella/mammalian-microsome (Ames) assay for the detection of mutagenic constituents in complex mixtures. Crude hazardous wastes and selected hazardous-waste extracts were fractionated on commercially available cellulose TLC plates. Mutagenicity testing was performed by applying a single overlay of minimal growth agar containing a tester strain of Salmonella and the optional metabolic activation system directly onto the developed chromatogram. Seven of 10 hazardous wastes demonstrated mutagenic activity when tested by the method. To assess the sensitivity of the modified TLC/Salmonella assay, 14 Salmonella mutagens from a wide range of chemical classes and polarities were tested. Eleven of the 14 mutagens were positive in the test system.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
- OSTI ID:
- 5783476
- Report Number(s):
- PB-88-107214/XAB; EPA-600/D-87/242
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HAZARDOUS MATERIALS
MUTAGEN SCREENING
SALMONELLA
THIN-LAYER CHROMATOGRAPHY
BIOASSAY
MUTAGENS
TOXICITY
WASTES
BACTERIA
CHROMATOGRAPHY
MATERIALS
MICROORGANISMS
SCREENING
SEPARATION PROCESSES
550300* - Cytology
560300 - Chemicals Metabolism & Toxicology