Minor antigen graft-versus-host reactions revealed in irradiated spleen and popliteal lymph node assays

Journal Article · Mon Oct 01 00:00:00 EDT 1984 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198410000-00015· OSTI ID:5777201

Claman, H N; Jaffee, B D

The graft-versus-hot (GVH) reaction across minor (non-H-2) histocompatibility barriers was studied in mice, in vivo. To increase GVH potential and to mimic clinical bone marrow transplantation protocols, we modified the popliteal lymph node (PLN) and the splenomegaly assays by irradiating the recipients before they received allogeneic lymphoid cell suspensions. In several combinations across major (H-2), minor (non-H-2) and multiple minor (non-H-2 plus minor lymphocyte stimulation) barriers, increased recipient organ weight (a measure of GVH activity) was seen with irradiated F1 recipients of parental cells. The irradiated splenomegaly (x-splenomegaly) assay was more sensitive than the (x-PLN) assay, but both correlated with in vivo GVH experiments of the P----F1 variety. The x-splenomegaly test indicated histoincompatibility in a system (B10.D2----BALB/c) in which the primary in vitro mixed leukocyte reactions is nonreactive, but in which systemic GVH can be induced. The x-splenomegaly test should be useful in analyzing complex reactions involving minor histocompatibility antigens in vivo.

• Research Organization: Univ. of Colorado School of Medicine, Denver
• OSTI ID: 5777201
• Journal Information: Transplantation; (United States), Vol. 38:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPH NODES
GRAFT-HOST REACTION
MICE
SPLEEN
ANTIGENS
BIOLOGICAL RADIATION EFFECTS
IN VIVO
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
SPLENOMEGALY
TRANSPLANTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
ORGANS
PATHOLOGICAL CHANGES
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SYMPTOMS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals