Gaseous oxide toxicity evaluated with cell monolayers on collagen-coated, gas-permeable teflon membranes
A system was developed to evaluate the cytotoxic potential of gaseous oxides in vitro. Target cells were MRC-5 human lung fibroblasts cultivated as monolayers on gas-permeable, FEP-Teflon membranes. Membranes were secured in Chamber/Dishes with a 25 mm diameter well. To promote attachment of fibroblasts to the membranes, the latter were incubated in collagen (Vitrogen) solutions for 10 min prior to plating the cells. The collagen pretreatment was significantly more effective than poly-L-lysine, fetal calf serum, polybrene and bovine serum albumin. Several types (mouse and calf) of acid-soluble and alcohol-soluble collagen fractions were evaluated, and all of them promoted cell attachment with equivalent efficiency. Cells on membranes were exposed to gases in a Plexiglass chamber with a gas flow of 2L/min. Sulfur dioxide caused a marked loss in cell viability (as indicated by ATP content of the monolayer) after 30 min exposure to 0.01% and 0.005%. A level of 0.001% did not affect viability, and none of the levels tested caused a sloughing of the monolayer after 90 min. Nitrogen dioxide induced a more modest drop in cell viability after 30 min exposure to 0.1%, while 0.005% and 0.05% were nontoxic. No cell sloughing occurred with NO/sub 2/ exposures, and exposures to CO/sub 2/ at levels of 20% for 90 min were nontoxic. This system, with cell culture monolayers on gas-permeable Teflon membranes, is simple and convenient. As such, it has potential application to cytotoxicity evaluations with numerous gases. 18 references, 6 figures, 1 table.
- OSTI ID:
- 5776791
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 54
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON DIOXIDE
TOXICITY
FIBROBLASTS
VIABILITY
NITROGEN DIOXIDE
SULFUR DIOXIDE
CELL CULTURES
CONTROLLED ATMOSPHERES
EXPERIMENTAL DATA
IN VITRO
LUNGS
MAN
MEASURING METHODS
SAMPLE PREPARATION
ANIMAL CELLS
ANIMALS
ATMOSPHERES
BODY
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DATA
INFORMATION
MAMMALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NUMERICAL DATA
ORGANS
OXIDES
OXYGEN COMPOUNDS
PRIMATES
RESPIRATORY SYSTEM
SOMATIC CELLS
SULFUR COMPOUNDS
SULFUR OXIDES
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)