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Title: Murine eosinophils labeled with indium-111 oxine: localization to delayed hypersensitivity reactions against a schistosomal antigen and to lymphokine in vivo

Abstract

We have evaluated a method for quantitation of eosinophil migration to stimuli in vivo. Upon transfusion into normal syngeneic mice, 111In-labeled eosinophils had an intravascular half-life of 9.5 hr and distributed predominantly into spleen, bone marrow, and liver. In either Schistosoma mansoni-infected mice or recipients of lymphoid cells from infected mice, intradermal (ear pinna) injection of the schistosomal egg antigenic preparation (SEA) elicited time-dependent accumulation of 111In-labeled eosinophils detectable by either gamma scintillation counting of tissue samples or by nuclear medicine external imaging. Intradermal administration of a lymphokine fraction (containing eosinophil stimulation promoter activity) similarly caused accumulation of 111In-labeled eosinophils. Both reactions depended on the concentration of stimulus (SEA or lymphokine). 111In-labeled neutrophils or macrophages or 125I-albumin did not preferentially accumulate at the reactions examined to the extent found with 111In-labeled eosinophils, indicating that localization of label depends on an active process and is due to eosinophils rather than a contaminating cell type. The method was used to estimate how long eosinotactic lymphokine remained at dermal sites: 60% of initial activity was present 12 hr after injection. The model is discussed with regard to the role of lymphokines in hypersensitivity reactions with eosinophil involvement, such as the granulomatous response tomore » S. mansoni eggs.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Department of Microbiology, Vanderbilt University School of Medicine, Nashville, TN
OSTI Identifier:
5776788
Resource Type:
Journal Article
Journal Name:
Blood; (United States)
Additional Journal Information:
Journal Volume: 61:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EOSINOPHILS; IMMUNE REACTIONS; INDIUM 111; TRACER TECHNIQUES; SCHISTOSOMA; BONE MARROW; LABELLED COMPOUNDS; LIVER; MICE; SPLEEN; TISSUE DISTRIBUTION; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; GLANDS; HELMINTHS; HEMATOPOIETIC SYSTEM; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PLATYHELMINTHS; RADIOISOTOPES; RODENTS; TISSUES; TREMATODES; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Rand, T H, Clanton, J A, Runge, V, English, D, and Colley, D G. Murine eosinophils labeled with indium-111 oxine: localization to delayed hypersensitivity reactions against a schistosomal antigen and to lymphokine in vivo. United States: N. p., 1983. Web.
Rand, T H, Clanton, J A, Runge, V, English, D, & Colley, D G. Murine eosinophils labeled with indium-111 oxine: localization to delayed hypersensitivity reactions against a schistosomal antigen and to lymphokine in vivo. United States.
Rand, T H, Clanton, J A, Runge, V, English, D, and Colley, D G. 1983. "Murine eosinophils labeled with indium-111 oxine: localization to delayed hypersensitivity reactions against a schistosomal antigen and to lymphokine in vivo". United States.
@article{osti_5776788,
title = {Murine eosinophils labeled with indium-111 oxine: localization to delayed hypersensitivity reactions against a schistosomal antigen and to lymphokine in vivo},
author = {Rand, T H and Clanton, J A and Runge, V and English, D and Colley, D G},
abstractNote = {We have evaluated a method for quantitation of eosinophil migration to stimuli in vivo. Upon transfusion into normal syngeneic mice, 111In-labeled eosinophils had an intravascular half-life of 9.5 hr and distributed predominantly into spleen, bone marrow, and liver. In either Schistosoma mansoni-infected mice or recipients of lymphoid cells from infected mice, intradermal (ear pinna) injection of the schistosomal egg antigenic preparation (SEA) elicited time-dependent accumulation of 111In-labeled eosinophils detectable by either gamma scintillation counting of tissue samples or by nuclear medicine external imaging. Intradermal administration of a lymphokine fraction (containing eosinophil stimulation promoter activity) similarly caused accumulation of 111In-labeled eosinophils. Both reactions depended on the concentration of stimulus (SEA or lymphokine). 111In-labeled neutrophils or macrophages or 125I-albumin did not preferentially accumulate at the reactions examined to the extent found with 111In-labeled eosinophils, indicating that localization of label depends on an active process and is due to eosinophils rather than a contaminating cell type. The method was used to estimate how long eosinotactic lymphokine remained at dermal sites: 60% of initial activity was present 12 hr after injection. The model is discussed with regard to the role of lymphokines in hypersensitivity reactions with eosinophil involvement, such as the granulomatous response to S. mansoni eggs.},
doi = {},
url = {https://www.osti.gov/biblio/5776788}, journal = {Blood; (United States)},
number = ,
volume = 61:4,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 1983},
month = {Fri Apr 01 00:00:00 EST 1983}
}