Increased mutagenicity of chromium compounds by nitrilotriacetic acid

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860070207· OSTI ID:5776324

Loprieno, N; Boncristiani, G; Venier, P; Montaldi, A; Majone, F; Bianchi, V; Paglialunga, S; Levis, A G

Nitrilotriacetic acid trisodium salt (NTA), which is a substitute for polyphosphates in household laundry detergents, and N-nitrosoiminodiacetic acid (NIDA), a derivative of NTA produced by metabolism of soil microorganisms, were tested for in vitro mutagenicity in bacteria and yeasts. No gene reversions in five strains of Salmonella typhimurium (TA1535, TA1537, TA1538, TA98, and TA100), no forward gene mutations in Schizosaccharomyces pombe P1, and no mitotic gene conversions at two loci in Saccharomyces cerevisiae D4 were induced by NTA and NIDA independently of the presence of rat liver metabolic activation. The influence of NTA on the mutagenic and clastogenic activity of several chromium compounds was examined in the Salmonella/microsome assay and in the sister chromatid exchange (SCE) assay in mammalian cell cultures (Chinese hamster ovary (CHO) line). NTA does not affect the genetic inactivity of water-soluble Cr(III) (Cr/sub 2/(SO/sub 4/)/sub 3/) and the direct mutagenicity of soluble Cr(VI) (Na/sub 2/CrO/sub 4/, K/sub 2/Cr/sub 2/O/sub 7/) compounds. The very insoluble Cr(VI) compounds PbCrO/sub 4/ and PbCrO/sub 4/ x PbO are instead clearly mutagenic in the Salmonella/microsome assay (TA100 strain) only in the presence of NTA or NaOH. The chromosome-damaging activity of PbCrO/sub 4/ is significantly increased by NTA but not by NaOH.

• Research Organization: Univ. of Pisa, Italy
• OSTI ID: 5776324
• Journal Information: Environ. Mutagen.; (United States), Vol. 7:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHROMATES
GENETIC EFFECTS
NTA
CHO CELLS
LEAD COMPOUNDS
MUTAGENESIS
REVERTANTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SALMONELLA TYPHIMURIUM
SISTER CHROMATID EXCHANGES
AMINO ACIDS
ANIMAL CELLS
BACTERIA
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM COMPOUNDS
CHROMOSOMAL ABERRATIONS
FUNGI
MICROORGANISMS
MUTANTS
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
SALMONELLA
TRANSITION ELEMENT COMPOUNDS
YEASTS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)