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Title: Increased mutagenicity of chromium compounds by nitrilotriacetic acid

Abstract

Nitrilotriacetic acid trisodium salt (NTA), which is a substitute for polyphosphates in household laundry detergents, and N-nitrosoiminodiacetic acid (NIDA), a derivative of NTA produced by metabolism of soil microorganisms, were tested for in vitro mutagenicity in bacteria and yeasts. No gene reversions in five strains of Salmonella typhimurium (TA1535, TA1537, TA1538, TA98, and TA100), no forward gene mutations in Schizosaccharomyces pombe P1, and no mitotic gene conversions at two loci in Saccharomyces cerevisiae D4 were induced by NTA and NIDA independently of the presence of rat liver metabolic activation. The influence of NTA on the mutagenic and clastogenic activity of several chromium compounds was examined in the Salmonella/microsome assay and in the sister chromatid exchange (SCE) assay in mammalian cell cultures (Chinese hamster ovary (CHO) line). NTA does not affect the genetic inactivity of water-soluble Cr(III) (Cr/sub 2/(SO/sub 4/)/sub 3/) and the direct mutagenicity of soluble Cr(VI) (Na/sub 2/CrO/sub 4/, K/sub 2/Cr/sub 2/O/sub 7/) compounds. The very insoluble Cr(VI) compounds PbCrO/sub 4/ and PbCrO/sub 4/ x PbO are instead clearly mutagenic in the Salmonella/microsome assay (TA100 strain) only in the presence of NTA or NaOH. The chromosome-damaging activity of PbCrO/sub 4/ is significantly increased by NTA but not by NaOH.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Pisa, Italy
OSTI Identifier:
5776324
Resource Type:
Journal Article
Journal Name:
Environ. Mutagen.; (United States)
Additional Journal Information:
Journal Volume: 7:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHROMATES; GENETIC EFFECTS; NTA; CHO CELLS; LEAD COMPOUNDS; MUTAGENESIS; REVERTANTS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SALMONELLA TYPHIMURIUM; SISTER CHROMATID EXCHANGES; AMINO ACIDS; ANIMAL CELLS; BACTERIA; BIOLOGICAL EFFECTS; CARBOXYLIC ACIDS; CHELATING AGENTS; CHROMIUM COMPOUNDS; CHROMOSOMAL ABERRATIONS; FUNGI; MICROORGANISMS; MUTANTS; MUTATIONS; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PLANTS; SALMONELLA; TRANSITION ELEMENT COMPOUNDS; YEASTS; 560301* - Chemicals Metabolism & Toxicology- Cells- (-1987); 560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)

Citation Formats

Loprieno, N, Boncristiani, G, Venier, P, Montaldi, A, Majone, F, Bianchi, V, Paglialunga, S, and Levis, A G. Increased mutagenicity of chromium compounds by nitrilotriacetic acid. United States: N. p., 1985. Web. doi:10.1002/em.2860070207.
Loprieno, N, Boncristiani, G, Venier, P, Montaldi, A, Majone, F, Bianchi, V, Paglialunga, S, & Levis, A G. Increased mutagenicity of chromium compounds by nitrilotriacetic acid. United States. https://doi.org/10.1002/em.2860070207
Loprieno, N, Boncristiani, G, Venier, P, Montaldi, A, Majone, F, Bianchi, V, Paglialunga, S, and Levis, A G. 1985. "Increased mutagenicity of chromium compounds by nitrilotriacetic acid". United States. https://doi.org/10.1002/em.2860070207.
@article{osti_5776324,
title = {Increased mutagenicity of chromium compounds by nitrilotriacetic acid},
author = {Loprieno, N and Boncristiani, G and Venier, P and Montaldi, A and Majone, F and Bianchi, V and Paglialunga, S and Levis, A G},
abstractNote = {Nitrilotriacetic acid trisodium salt (NTA), which is a substitute for polyphosphates in household laundry detergents, and N-nitrosoiminodiacetic acid (NIDA), a derivative of NTA produced by metabolism of soil microorganisms, were tested for in vitro mutagenicity in bacteria and yeasts. No gene reversions in five strains of Salmonella typhimurium (TA1535, TA1537, TA1538, TA98, and TA100), no forward gene mutations in Schizosaccharomyces pombe P1, and no mitotic gene conversions at two loci in Saccharomyces cerevisiae D4 were induced by NTA and NIDA independently of the presence of rat liver metabolic activation. The influence of NTA on the mutagenic and clastogenic activity of several chromium compounds was examined in the Salmonella/microsome assay and in the sister chromatid exchange (SCE) assay in mammalian cell cultures (Chinese hamster ovary (CHO) line). NTA does not affect the genetic inactivity of water-soluble Cr(III) (Cr/sub 2/(SO/sub 4/)/sub 3/) and the direct mutagenicity of soluble Cr(VI) (Na/sub 2/CrO/sub 4/, K/sub 2/Cr/sub 2/O/sub 7/) compounds. The very insoluble Cr(VI) compounds PbCrO/sub 4/ and PbCrO/sub 4/ x PbO are instead clearly mutagenic in the Salmonella/microsome assay (TA100 strain) only in the presence of NTA or NaOH. The chromosome-damaging activity of PbCrO/sub 4/ is significantly increased by NTA but not by NaOH.},
doi = {10.1002/em.2860070207},
url = {https://www.osti.gov/biblio/5776324}, journal = {Environ. Mutagen.; (United States)},
number = ,
volume = 7:2,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1985},
month = {Tue Jan 01 00:00:00 EST 1985}
}