Linear relationship between lethal mutation yield and intake of ethyl methanesulfonate in Drosophila melanogaster
Males of Drosophila melanogaster were fed sucrose solutions containing various concentrations of EMS (from 0.25 to 10mM) for 24 hr. To measure the intake of an EMS solution, /sup 3/H-labeled sucrose was added to the feeding solution, and the /sup 3/H activity inside the flies was used as a measure for the intake volume of EMS solution. The relationship between the estimated absorbed dose and the exposure concentration was almost linear in a low concentration range but became concave with a downward curvature in a high concentration range. The dose-response relationship between the frequency of sex-linked recessive lethals and the estimated absorbed dose showed no deviation from linearity at all the five absorbed doses tested. It may be concluded that the absorbed doses thus estimated were very close to true absorbed doses, indicating the usefulness of the present method for dosimetry of chemicals to be given to files.
- Research Organization:
- Nagasaki Univ. School of Medicine, Japan
- OSTI ID:
- 5775138
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 7:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EMS
INGESTION
TOXICITY
MUTATION FREQUENCY
DOSE-RESPONSE RELATIONSHIPS
DROSOPHILA
LETHAL MUTATIONS
MALES
RECESSIVE MUTATIONS
SACCHAROSE
TRITIUM COMPOUNDS
ANIMALS
ARTHROPODS
CARBOHYDRATES
DIPTERA
DISACCHARIDES
ESTERS
FLIES
FRUIT FLIES
INSECTS
INTAKE
INVERTEBRATES
LABELLED COMPOUNDS
MUTAGENS
MUTATIONS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SACCHARIDES
SULFONIC ACID ESTERS
560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)