Follow-up study on histogenesis of microcephaly associated with ectopic gray matter induced by prenatal {gamma}-irradiation in the mouse
- Nagoya Univ. (Japan); and others
Brain malformation with ectopic gray matter was visualized with magnetic resonance imaging in small-sized heads of prenatally exposed atomic bomb survivors. The identical brain malformation was reproduced in mice and its histogenesis was studied in the present experiment. Pregnant mice were exposed to {sup 60}Co {gamma}-irradiation at a single dose of 1.5 Gy on embryonic day 13 (E13), and then injected intraperitoneally with 30 mg/kg BrdU on E15. The extensive dead cells appeared throughout the brain mantle at 6 hours (h) after exposure. On E16 cell aggregations formed rosettes. On E18 a high proportion of BrdU-labeled cells reached the superficial layers of the cortical plate with the remaining cells located in the ectopic neuronal masses. The quantitative study showed that labeled cells in layers II to III were fewer and those in layers IV to VI more numerous in the prenatally irradiated adult mice than in controls. The anti-GFAP immunostaining revealed that the glial fibers in the irradiated mice were preserved, but disorganized. These findings suggested that the majority of migrating neurons were able to arrive at their normal layers, but some neurons remained due to the interrupted migratory pathway and eventually formed ectopic neuronal masses beneath the subcortical white matter. 60 refs., 5 figs., 1 tab.
- OSTI ID:
- 577004
- Journal Information:
- Journal of Neuropathology and Experimental Neurology, Vol. 55, Issue 3; Other Information: PBD: Mar 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
APPLIED STUDIES
55 BIOLOGY AND MEDICINE
BASIC STUDIES
BRAIN
BIOLOGICAL RADIATION EFFECTS
MALFORMATIONS
SPATIAL DOSE DISTRIBUTIONS
A-BOMB SURVIVORS
FETUSES
MENTAL DISORDERS
PRENATAL IRRADIATION
BIOLOGICAL MODELS
PRENATAL EXPOSURE
ANIMAL CELLS
NMR IMAGING
MICE
COBALT 60
BUDR
IONIZING RADIATIONS
HISTOLOGY