Role of calcium in effects of atrial natriuretic peptide on aldosterone production in adrenal glomerulosa cells

Journal Article · Wed Apr 01 00:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5768396

Chartier, L; Schiffrin, E L

Atrial natriuretic peptide (ANP) inhibits the stimulation of aldosterone secretion by isolated adrenal glomerulosa cells produced by angiotensin II (ANG II), ACTH, and potassium. The effect of ANP on the dose-response curve of aldosterone stimulated by ANG II, ACTH, and potassium on isolated rat adrenal glomerulosa cells was studied. In the presence of ANP the maximal response of aldosterone output stimulated by ANG II or potassium decreased and the half-maximum (EC/sub 50/) of the response to ACTH was displaced to the right. Because these effects resemble those of calcium-channel blockers, the authors investigated the effect of different concentrations of nifedipine, a dihydropyridine calcium-channel blocker, on the dose-response curve of aldosterone stimulated by ANG II, ACTH, and potassium. Nifedipine produced effects similar to ANP. The maximal response of aldosterone stimulated by ANG II and potassium was decreased and the dose-response curve to ACTH was displaced to the right. ANP decreased the maximal response of aldosterone to the dihydropyridine derivative BAY K8644, a calcium-channel activator, without change in its EC/sub 50/. In contrast, nifedipine displaced the dose-response curve to BAY K8644 to the right as expected of a competitive inhibitor. The effect of ANP and nifedipine on basal and stimulated /sup 45/Ca influx into isolated rat adrenal glomerulosa cells was studied. ANP may act on the rat adrenal glomerulosa cells at least in part by interference with calcium entry.

• Research Organization: Clinical Research Institute of Montreal, Quebec
• OSTI ID: 5768396
• Journal Information: Am. J. Physiol.; (United States), Vol. 252:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALDOSTERONE
BIOSYNTHESIS
CALCIUM 45
UPTAKE
PEPTIDES
BIOLOGICAL EFFECTS
ACTH
ADRENAL GLANDS
ANGIOTENSIN
CALCIUM CHLORIDES
DOSE-RESPONSE RELATIONSHIPS
SECRETION
ADRENAL HORMONES
ALDEHYDES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM COMPOUNDS
CALCIUM HALIDES
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CHLORIDES
CHLORINE COMPOUNDS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
GLOBULINS
HALIDES
HALOGEN COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
KETONES
MINERALOCORTICOIDS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
PROTEINS
RADIOISOTOPES
STEROIDS
SYNTHESIS
VASOCONSTRICTORS
550201* - Biochemistry- Tracer Techniques