Biosynthesis of thiamine triphosphate and identification of thiamine diphosphate-binding proteins of rat liver hyaloplasm

Journal Article · Mon Mar 10 00:00:00 EST 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5767682

Voskoboev, A I; Chernikevich, I P

The nature of the proteins of rat liver hyaloplasm that bind ThDP* was studied. When injection of (/sup 14/C)thiamine was used as the marker, an electrophoretically homogeneous protein preparations, containing (/sup 14/C)ThDP, identified as transketolase, was isolated from the soluble fraction of the liver. No other nonenzymatic proteins that bind ThDP and might serve as the substrate in the synthesis of ThTP were detected in the hyaloplasm. It was shown that transfer of the phosphate group by rat liver ThDP kinase occurs to the free ThDP, and not to protein-bound ThDP, as was previously asserted.

• OSTI ID: 5767682
• Journal Information: Biochemistry (Engl. Transl.); (United States), Vol. 50:9; Other Information: Translated from Biokhimiya; 50: No. 9, 1421-1427(Sep 1985)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COENZYMES
BIOSYNTHESIS
CYTOPLASM
BIOCHEMISTRY
RADIOENZYMATIC ASSAY
RADIORECEPTOR ASSAY
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PROTEINS
THIAMINE
CARBON 14 COMPOUNDS
CATALYTIC EFFECTS
ELECTROPHORESIS
LABELLING
LIVER
LIVER CELLS
PHOSPHORUS COMPOUNDS
RATS
AMINES
ANIMAL CELLS
ANIMALS
AZINES
AZOLES
BODY
CELL CONSTITUENTS
CHEMISTRY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PYRIMIDINES
RODENTS
SOMATIC CELLS
SYNTHESIS
THIAZOLES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques