Characteristics of some fermentative bacteria from a thermophilic methane-producing fermenter
Anaerobic bacteria from a 55/sup 0/C methane-producing beef waste fermenter were enumerated, isolated, and characterized. Direct microscopic bacterial counts were 5.2-6.8 X 10/sup 10/ per g fermenter effluent. Using a nonselective roll-tube medium which contained 40% fermenter effluent, 8.5-14.1% of microscopic count was culturable. Deletion of fermenter effluent significantly reduced the viable count. Sixty-four randomly picked strains were characterized. All were pleomorphic, gram-negative, anaerobic rods, many of which were difficult to grow in liquid media. The strains were divided into 5 major groups based on glucose fermentation, hydrogen sulfide production, starch hydrolysis, fermentation products, and morphology. Glucose was fermented by 75% of the isolates, 76% utilized starch, 25% produced hydrogen sulfide, 76% produced hydrogen, 37% produced indole, 21% hydrolyzed gelatin, and 13% were sporeformers. Ethanol, lactate, formate, acetate, and hydrogen were common fermentation products. Twenty-four representative strains had 1-12 flagella. Growth was observed between 35 and 73/sup 0/C. These studies indicate that species diversity among the isolated organisms was low. 38 references, 3 tables.
- Research Organization:
- Dept. of Agriculture, Clay Center, NE
- OSTI ID:
- 5764073
- Journal Information:
- Microb. Ecol.; (United States), Vol. 10:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIA
SPECIES DIVERSITY
MANURES
ANAEROBIC DIGESTION
METHANE
BIOSYNTHESIS
BIOREACTORS
CLASSIFICATION
EXPERIMENTAL DATA
PRODUCTIVITY
THERMOPHILIC CONDITIONS
AGRICULTURAL WASTES
ALKANES
BIOCONVERSION
DATA
DIGESTION
HYDROCARBONS
INFORMATION
MANAGEMENT
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC WASTES
PROCESSING
SYNTHESIS
WASTE MANAGEMENT
WASTE PROCESSING
WASTES
090122* - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)