DNA modification in vivo by derivatives of glucose: Enhancement by glutathione depletion

Journal Article · Sat Dec 01 00:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:5762997

Shires, T K; Tresnak, J; Kaminsky, M; Herzog, S L; Truc-Pham, B ^[1]

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1. Univ. of Iowa, Iowa City (USA)

When BHK or HTC cells are cultured for 20 min with (U-{sup 14}C)glucose in the presence of agents that deplete reduced glutathione, DNA banded from the cells in cesium salt gradients containing guanidium HCl is radioactively labeled. This depletion-dependent labeling required live cells. It was not caused by reactive contaminants in the radioactive glucose preparations, by carbohydrate or protein comigration into the DNA band, or by metabolism of glucose into deoxyribose. Labeling levels are similar whether depletion is achieved by oxidation (with the drug diamide) or by inhibition of synthesis (with methionine sulfoximine). A temporal association between GSH repletion and the appearance of D-lactate, the putative unique product of GSH-dependent glyoxylase action on pyruvaldehyde, suggests possible involvement of 3-carbon dicarbonyls.

• OSTI ID: 5762997
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Vol. 4:15; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
ALKYLATION
ALDEHYDES
CARBON 14 COMPOUNDS
GLUTATHIONE
LACTIC ACID
TRACER TECHNIQUES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DRUGS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
550201* - Biochemistry- Tracer Techniques