Reproducibility of microbial mutagenicity assays. I. Tests with Salmonella typhimurium and Escherichia coli using a standardized protocol

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Environ. Manage.; (United States)

OSTI ID:5762937

Dunkel, V C; Zeiger, E; Brusick, D; McCoy, E; McGregor, D; Mortelmans, K; Rosenkranz, H S; Simmon, V F

The Salmonella/microsome test developed by Ames and his coworkers has been widely used in the evaluation of chemicals for genotoxic potential. Although the value of this assay is well recognized, there have been no comprehensive studies on the interlaboratory reproducibility of the method using a standardized protocol. A program was therefore initiated to compare the results obtained in four laboratories from testing a series of coded mutagens and nonmutagens using a standardized protocol. Additional objectives of this study were to compare male Fisher 344 rat, B6C3F1 mouse, and Syrian hamster liver S-9 preparations for the activation of chemicals; to compare Aroclor 1254-induced liver S-9 from all three species with the corresponding non-induced liver S-9's; and to compare the response of Escherichia coli WP-2 uvrA with the Salmonella typhimurium tester strains recommended by Ames. Since a primary use of in vitro microbial mutagenesis tests is the identification of potential carcinogens by their mutagenicity, the authors decided to compare the animal species and strains used by the National Cancer Institute/National Toxicology Program (NCI/NTP) for animal carcinogenicity studies.

• Research Organization: National Cancer Inst., Bethesda, MD
• OSTI ID: 5762937
• Journal Information: Environ. Manage.; (United States), Vol. 6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOASSAY
INTERLABORATORY COMPARISONS
STANDARDIZATION
CARCINOGENS
MUTAGEN SCREENING
ESCHERICHIA COLI
HAMSTERS
MICE
RATS
SALMONELLA TYPHIMURIUM
ANIMALS
BACTERIA
MAMMALS
MICROORGANISMS
RODENTS
SALMONELLA
SCREENING
VERTEBRATES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)