Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts
Abstract
This research examined the titers of hexokinase (HK), phosphofructokinase (PFK), and xylulokinase (XUK) in Saccharomyces cerevisiae and two xylose fermenting yeasts, Pachysolen tannophilus and Candida shehatae, following shifts in carbon source and aeration. Xylose-grown C. shehatae, glucose-grown P. tannophilus, and glucose-grown S. cerevisiae, had the highest specific activities of XUK, HK, and PFK, respectively. XUK was induced by xylose to moderate levels in both P. tannophilus and C. shehatae, but was present only in trace levels in S. cerevisiae. HK activities in P. tannophilus were two to three fold higher when cells were grown on glucose than when grown on xylose, but HK levels were less inducible in C. shehatae. The PFK activities in S. cerevisiae were 1.5 to 2 times higher than in the two xylose-fermenting yeasts. Transfer from glucose to xylose rapidly inactivated HK in P. tannophilus, and transfer from xylose to glucose inactivated XUK in C. shehatae. The patterns of induction and inactivation indicate that the basic regulatory mechanisms differ in the two xylose fermenting yeasts. 42 refs., 2 figs., 4 tabs.
- Authors:
-
- Forest Products Lab., Madison, WI (United States)
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 576212
- Report Number(s):
- CONF-960539-
Journal ID: ABIBDL; ISSN 0273-2289; TRN: 98:000980-0006
- Resource Type:
- Journal Article
- Journal Name:
- Applied Biochemistry and Biotechnology
- Additional Journal Information:
- Journal Volume: 63-65; Conference: 18. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 5-9 May 1996; Other Information: PBD: Spr 1997
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; AERATION; CANDIDA; GLUCOSE; HEXOKINASE; PHOSPHOTRANSFERASES; SACCHAROMYCES CEREVISIAE; XYLOSE; YEASTS; FERMENTATION; BIOMASS; ENZYME ACTIVITY
Citation Formats
Yang, V W, and Jeffries, T W. Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts. United States: N. p., 1997.
Web. doi:10.1007/BF02920416.
Yang, V W, & Jeffries, T W. Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts. United States. https://doi.org/10.1007/BF02920416
Yang, V W, and Jeffries, T W. 1997.
"Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts". United States. https://doi.org/10.1007/BF02920416.
@article{osti_576212,
title = {Regulation of phosphotransferases in glucose- and xylose-fermenting yeasts},
author = {Yang, V W and Jeffries, T W},
abstractNote = {This research examined the titers of hexokinase (HK), phosphofructokinase (PFK), and xylulokinase (XUK) in Saccharomyces cerevisiae and two xylose fermenting yeasts, Pachysolen tannophilus and Candida shehatae, following shifts in carbon source and aeration. Xylose-grown C. shehatae, glucose-grown P. tannophilus, and glucose-grown S. cerevisiae, had the highest specific activities of XUK, HK, and PFK, respectively. XUK was induced by xylose to moderate levels in both P. tannophilus and C. shehatae, but was present only in trace levels in S. cerevisiae. HK activities in P. tannophilus were two to three fold higher when cells were grown on glucose than when grown on xylose, but HK levels were less inducible in C. shehatae. The PFK activities in S. cerevisiae were 1.5 to 2 times higher than in the two xylose-fermenting yeasts. Transfer from glucose to xylose rapidly inactivated HK in P. tannophilus, and transfer from xylose to glucose inactivated XUK in C. shehatae. The patterns of induction and inactivation indicate that the basic regulatory mechanisms differ in the two xylose fermenting yeasts. 42 refs., 2 figs., 4 tabs.},
doi = {10.1007/BF02920416},
url = {https://www.osti.gov/biblio/576212},
journal = {Applied Biochemistry and Biotechnology},
number = ,
volume = 63-65,
place = {United States},
year = {Wed Dec 31 00:00:00 EST 1997},
month = {Wed Dec 31 00:00:00 EST 1997}
}