Measuring chlorophyll a and /sup 14/C-labeled photosynthate in aquatic angiosperms by the use of a tissue solubilizer
A compound that quantitatively correlated with chlorophyll a could be measured fluorometrically in the extracts of leaves of three aquatic angiosperms (Myriophyllum heterophyllum Michx., Potamogeton crispus L., Elodea canadensis Michx.) treated with the tissue solubilizer BTS-450. Fluorescent characteristics of the solubilized plant tissues were stable for several weeks in the dark at temperatures up to 60/sup 0/C but rapidly degraded in sunlight or when acidified. /sup 14/C-Labeled photosynthate, which had been fixed by leaf discs during 1- to 10-hour exposure to H/sup 14/CO/sub 3/, was also readily extracted by the tissue solubilizer. Solubilizer extraction can, therefore, be use to determine both chlorophyll a content and /sup 14/C incorporation rates in the same leaf sample. The method is practical, because no grinding is required, the fluorescent characteristics of the extracts are stable, and analyses can be performed with very little plant material (about 3 milligrams).
- Research Organization:
- Michigan State Univ., Hickory Corners
- OSTI ID:
- 5761268
- Journal Information:
- Plant Physiol.; (United States), Vol. 69:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHLOROPHYLL
QUANTITATIVE CHEMICAL ANALYSIS
PHOTOSYNTHESIS
TRACER TECHNIQUES
AQUATIC ORGANISMS
CARBON 14 COMPOUNDS
SEPARATION PROCESSES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
SYNTHESIS
550501* - Metabolism- Tracer Techniques
550200 - Biochemistry