The response of human and rodent cells to hyperthermia
- College of Physicians and Surgeons of Columbia University, New York, NY (USA)
Inherent cellular radiosensitivity in vitro has been shown to be a good predictor of human tumor response in vivo. In contrast, the importance of the intrinsic thermosensitivity of normal and neoplastic human cells as a factor in the responsiveness of human tumors to adjuvant hyperthermia has never been analyzed systematically. A comparison of thermal sensitivity and thermo-radiosensitization in four rodent and eight human-derived cell lines was made in vitro. Arrhenius plots indicated that the rodent cells were more sensitive to heat killing than the human, and the break-point was 0.5 degrees C higher for the human than rodent cells. The relationship between thermal sensitivity and the interaction of heat with X rays at low doses was documented by thermal enhancement ratios (TER's). Cells received either a 1 hr exposure to 43 degrees C or a 20 minute treatment at 45 degrees C before exposure to 300 kVp X rays. Thermal enhancement ratios ranged from 1.0 to 2.7 for human cells heated at 43 degrees C and from 2.1 to 5.3 for heat exposures at 45 degrees C. Thermal enhancement ratios for rodent cells were generally 2 to 3 times higher than for human cells, because of the fact that the greater thermosensitivity of rodent cells results in a greater enhancement of radiation damage. Intrinsic thermosensitivity of human cells has relevance to the concept of thermal dose; intrinsic thermo-radiosensitization of a range of different tumor cells is useful in documenting the interactive effects of radiation combined with heat.
- OSTI ID:
- 5760205
- Journal Information:
- International Journal of Radiation Oncology, Biology and Physics; (USA), Vol. 20:4; ISSN 0360-3016
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEAT
RADIOSENSITIVITY EFFECTS
TUMOR CELLS
RADIOSENSITIVITY
HYPERTHERMIA
MAN
RODENTS
X RADIATION
ANIMAL CELLS
ANIMALS
BODY TEMPERATURE
ELECTROMAGNETIC RADIATION
ENERGY
IONIZING RADIATIONS
MAMMALS
PRIMATES
RADIATIONS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture