Benzo(A)pyrene (BaP) treatment results in complete infertility in female pigeons
- Masonic Medical Research Lab., Utica, NY (United States)
BaP is a carcinogenic polycyclic aromatic hydrocarbon (PAH) and a common environmental pollutant. Show Racer and White Carneau female pigeons injected weekly with BaP for 3 for 5 months were completely infertile, with ovaries appearing necrotic or oxidized. Fertility in benzo(e)pyrene (BeP, a noncarcinogenic PAH) treated birds was the same as for corn oil treated controls, as was embryo development. Thus, infertility in BaP treated birds appears to be related to its structure-carcinogenic potential. There was no readily apparent affect of BaP treatment on testes from male birds. In order to determine whether BaP metabolites covalently bind to DNA in the ovaries of these birds, pigeons were injected with BaP or BeP, controls were injected with corn oil. Animals were sacrificed 24h later, the ovaries or testes removed, and the DNA isolated and analyzed for PAH-DNA adducts by {sup 32}P-post labeling assay. One major and one minor PAH-DNA adduct was found in ovaries and testes from BaP treated birds. However, no PAH adducts were found in BeP treated or control animals. Thus, problems with fertility may arise because of the alteration in DNA by BaP metabolite binding in ovaries where rapid cell growth occurs during egg production.
- OSTI ID:
- 5751761
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BENZOPYRENE
TOXICITY
DNA ADDUCTS
BIOSYNTHESIS
PIGEONS
FERTILITY
METABOLISM
METABOLITES
OVARIES
PHOSPHORUS 32
REPRODUCTION
TESTES
TRACER TECHNIQUES
ADDUCTS
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
BODY
CONDENSED AROMATICS
DAYS LIVING RADIOISOTOPES
FEMALE GENITALS
GONADS
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MALE GENITALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology