A sensitive enzyme electrode for phenol monitoring
- GBF-Gesellschaft fuer Biotechnologische Forschung mbH, Braunschweig (West Germany)
Tyrosinase (EC.1.14.18.1) was immobilized onto graphite electrodes, which had been modified with tetracyanoquinodimethane (TCNQ). The response time, 12 or 35 s, was dependent on the enzyme immobilization technique used. The electrodes showed a linear calibration function up to 25 or 65 {mu}M phenol, and a sensitivity of 0.36 or 2.2 A/M was achieved which was also dependent on the enzyme immobilization technique used. The detection limit for phenol was 0.23 {mu}M. The electrodes acted from potentials of {minus}200 to +180 mV (vs. a saturated Ag/AgCl electrode). The electrode signal was independent of pH within the pH range 4.5-6.0. The enzyme electrode responded to phenol (100%), p-cresol (93%) and catechol (330%), but not to o-cresol and L-tyrosine. The electrodes showed a stability for more than one week. The electrodes can be utilized for the sensitive assay of phenol in water.
- OSTI ID:
- 5745737
- Journal Information:
- Analytical Letters; (United States), Vol. 23:4; ISSN 0003-2719
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PHENOL
QUANTITATIVE CHEMICAL ANALYSIS
CALIBRATION
CHEMICAL REACTION KINETICS
ELECTRODES
EXPERIMENTAL DATA
GRAPHITE
IMMOBILIZED ENZYMES
OXIDATION
PH VALUE
WATER
AROMATICS
CARBON
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DATA
ELEMENTAL MINERALS
ELEMENTS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
KINETICS
MINERALS
NONMETALS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHENOLS
REACTION KINETICS
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
400102 - Chemical & Spectral Procedures