Use of denatured radioalbumin for determination of trypsin and chymotrypsin inhibitors in different plant seeds
Journal Article
·
· Journal of Agricultural and Food Chemistry; (United States)
- Univ. of Veterinary Medicine, Kosice (Czechoslovakia)
The procedure for determination of trypsin and chymotrypsin inhibitors with urea-denatured albumin labeled by {sup 125}I is described. The content of both types of inhibitory activity has been determined in crude extracts of soybean, bean, lentil, pea, horse bean, maize, and 20 pea cultivars. The method is sufficiently sensitive, reliable, and particularly suitable when estimations must be done in crude plant extract with low inhibitory activity.
- OSTI ID:
- 5745374
- Journal Information:
- Journal of Agricultural and Food Chemistry; (United States), Vol. 39:2; ISSN 0021-8561
- Country of Publication:
- United States
- Language:
- English
Similar Records
sup 1 H assignments and secondary structure determination of the soybean trypsin/chymotrypsin Bowman-Birk inhibitor
Structure-function relationships of proteinase from soybean (Bowman-Birk) and lima bean: modification by N-acetylimidazole
Structure-function relationships of proteinase inhibitors from soybean (Bowman-Birk) and lima bean
Journal Article
·
Tue Apr 09 00:00:00 EDT 1991
· Biochemistry; (United States)
·
OSTI ID:5745374
Structure-function relationships of proteinase from soybean (Bowman-Birk) and lima bean: modification by N-acetylimidazole
Technical Report
·
Sun Jan 01 00:00:00 EST 1978
·
OSTI ID:5745374
Structure-function relationships of proteinase inhibitors from soybean (Bowman-Birk) and lima bean
Journal Article
·
Sat Aug 25 00:00:00 EDT 1979
· J. Biol. Chem.; (United States)
·
OSTI ID:5745374
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ENZYME INHIBITORS
CHEMICAL ANALYSIS
ALBUMINS
CHYMOTRYPSIN
IODINE 125
LABELLED COMPOUNDS
LEGUMINOSAE
MAIZE
PLANTS
RELIABILITY
SEEDS
SENSITIVITY
TRACER TECHNIQUES
TRYPSIN
BETA DECAY RADIOISOTOPES
CEREALS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GRAMINEAE
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PROTEINS
RADIOISOTOPES
SERINE PROTEINASES
550201* - Biochemistry- Tracer Techniques
ENZYME INHIBITORS
CHEMICAL ANALYSIS
ALBUMINS
CHYMOTRYPSIN
IODINE 125
LABELLED COMPOUNDS
LEGUMINOSAE
MAIZE
PLANTS
RELIABILITY
SEEDS
SENSITIVITY
TRACER TECHNIQUES
TRYPSIN
BETA DECAY RADIOISOTOPES
CEREALS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GRAMINEAE
HYDROLASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PROTEINS
RADIOISOTOPES
SERINE PROTEINASES
550201* - Biochemistry- Tracer Techniques