Incorporation and distribution of dihomo-gamma-linolenic acid, arachidonic acid, and eicosapentaenoic acid in cultured human keratinocytes
Human keratinocytes in culture were labelled with /sup 14/C-dihomo-gamma-linolenic acid, /sup 14/C-arachidonic acid or /sup 14/C-eicosapentaenoic acid. All three eicosanoid precursor fatty acids were effectively incorporated into the cells. In phospholipids most of the radioactivity was recovered, in neutral lipids a substantial amount, and as free unesterified fatty acids only a minor amount. Most of the radioactivity was found in phosphatidylethanolamine which was also the major phospholipid as measured by phosphorous assay. The incorporation of dihomo-gamma-linolenic acid and arachidonic acid into lipid subfractions was essentially similar. Eicosapentaenoic acid was, however, much less effectively incorporated into phosphatidylinositol + phosphatidylserine and, correspondingly, more effectively into triacylglycerols as compared to the two other precursor fatty acids. Once incorporated, the distribution of all three precursor fatty acids was relatively stable, and only minor amounts of fatty acids were released into the culture medium during short term culture (two days). Our study demonstrates that eicosanoid precursor fatty acids are avidly taken up by human keratinocytes and esterified into membrane lipids. The clinical implication of this finding is that dietary manipulations might be employed to cause changes in the fatty acid composition of keratinocytes.
- Research Organization:
- Univ. of Turku, Finland
- OSTI ID:
- 5742948
- Journal Information:
- Prostaglandins; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Eicosapentaenoic and dihomo gamma linolenic acid metabolism by cultured rat mesangial cells
Metabolism of arachidonic and adrenic acids in molecular species of gycerophospholipids in mouse brain
Related Subjects
ARACHIDONIC ACID
ESTERIFICATION
MEMBRANE TRANSPORT
CARBOXYLIC ACIDS
LINOLENIC ACID
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CELL CULTURES
EPIDERMIS
KERATIN
PHOSPHOLIPIDS
PRECURSOR
TRACER TECHNIQUES
ANIMAL TISSUES
BODY
CHEMICAL REACTIONS
EPITHELIUM
ESTERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
SCLEROPROTEINS
SKIN
TISSUES
550201* - Biochemistry- Tracer Techniques