Determination and temperature effects of lidocaine (lignocaine) hydrochloride, epinephrine, methylparaben, 2,6-dimethylaniline, and p-hydroxybenzoic acid in USP lidocaine injection by ion-pair reversed-phase high pressure liquid chromatography

Journal Article · Fri May 01 00:00:00 EDT 1981 · J. Chromatogr. Sci.; (United States)

DOI:https://doi.org/10.1093/chromsci/19.5.253· OSTI ID:5742034

Smith, D J

USP Lidocaine injection was assayed using ion-pair high pressure liquid chromatography with an octylsilane (RP-8) reversed-phase column packing and a mobile phase consisting of D-10-camphorsulfonic acid/methanol/acetic acid/water. The effect of temperature was investigated to determine the optimum temperature for separating the drug components and their degradation products. Lidocaine (lignocaine) hydrochloride, epinephrine, methylparaben, and p-hydroxybenzoic acid were separated at 50 degrees C. 2,6-Dimethylaniline was separated from lidocaine at 15 degrees C. An aliquot of the sample was injected directly into the liquid chromatograph, and after separation the compounds were quantitated by their spectrophotometric response at 254 nm (lidocaine) or 280 nm (lidocaine plus epinephrine).

• OSTI ID: 5742034
• Journal Information: J. Chromatogr. Sci.; (United States), Vol. 19:5
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ADRENALINE
LIQUID COLUMN CHROMATOGRAPHY
SPECTRAL RESPONSE
AMINES
AROMATICS
ION PAIRS
TEMPERATURE EFFECTS
ADRENAL HORMONES
AUTONOMIC NERVOUS SYSTEM AGENTS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHROMATOGRAPHY
DRUGS
HORMONES
NEUROREGULATORS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
STEROID HORMONES
SYMPATHOMIMETICS
400102* - Chemical & Spectral Procedures