X-ray microanalysis of exocrine glands in animal models for cystic fibrosis

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Scanning Electron Microsc.; (United States)

OSTI ID:5740119

Mueller, R M.R.; Roomans, G M

Elemental distribution and ultrastructure of the submandibular gland, the parotid gland and the pancreas were investigated in three suggested animal models of the disease cystic fibrosis: the chronically reserpinized rat, the chronically isoproterenol-treated rat, and the chronically pilocarpine-treated rat. To elucidate the cellular mechanism underlying the effects of these treatments, chronic effects of specific alpha - and beta -adrenergic agonists, as well as acute effects of reserpine and various agonists were also investigated. Reserpine, isoproterenol, and pilocarpine cause an increase in the calcium concentration in submandibular gland acinar cells, due to an increased calcium content of the intracellular mucus. In the parotid gland, reserpine and isoproterenol cause a decrease of the calcium concentration in acinar cells, due to a lower calcium content of the zymogen granules. In the submandibular gland, a decreased cellular Na concentration was noted after chronic treatment with isoproterenol or pilocarpine, and after a single dose of reserpine or isoproterenol. Ultrastructural changes in the exocrine glands investigated included excessive accumulation of intracellular secretory material and formation of abnormal uncondensed secretion granules. A common pattern in the animal models appears to be (1) inhibition of secretion resulting in intracellular accumulation of secretory material, (2) synthesis of secretory macromolecules with altered cation-binding properties.

• Research Organization: Univ. of Stockholm, Sweden
• OSTI ID: 5740119
• Journal Information: Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ELECTRON MICROPROBE ANALYSIS
DIAGNOSTIC USES
PANCREAS
ULTRASTRUCTURAL CHANGES
SALIVARY GLANDS
BIOLOGICAL MODELS
PILOCARPINE
RATS
RESERPINE
RESPIRATORY SYSTEM DISEASES
SECRETION
ALKALOIDS
ANIMALS
ANTIHYPERTENSIVE AGENTS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BODY
CARDIOVASCULAR AGENTS
CHEMICAL ANALYSIS
DIGESTIVE SYSTEM
DISEASES
DRUGS
ENDOCRINE GLANDS
GLANDS
HETEROCYCLIC COMPOUNDS
INDOLES
MAMMALS
MICROANALYSIS
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PYRROLES
RODENTS
SYMPATHOLYTICS
USES
VERTEBRATES
550602* - Medicine- External Radiation in Diagnostics- (1980-)