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Title: Histochemical demonstration of mercury in the olfactory system of salmon (Salmo salar L. ) following treatments with dietary methylmercuric chloride and dissolved mercuric chloride

Abstract

The deposition of organic and inorganic mercury compounds was studied histochemically in the salmon (Salmo salar L.) olfactory system. One group of salmon was given fodder pellets containing methylmercuric chloride (CH{sub 3}HgCl, 99 micrograms Hg/g) for 4 weeks. Other groups of fish were exposed to dissolved mercuric chloride (HgCl{sub 2}, 270 micrograms Hg/liter) for 2, 6, and 12 hr, respectively. In both series of experiments, the radioisotope {sup 203}Hg was included in order to determine the accumulation of mercury in the olfactory system. Gamma-spectrometry showed that both mercury compounds accumulated in the olfactory rosettes and their nerves. Tissue sections from the rosettes and olfactory nerves were subjected to autometallographic silver enhancement, thereby rendering mercury deposits visible for light and electron microscopy. Microscopic analysis demonstrated an intense and comprehensive Hg deposition in the axons and Schwann cells of both methylmercury- and inorganic mercury-exposed fish. On the other hand, the two mercury compounds showed different staining patterns in the sensory epithelium. The silver grains evoked by methylmercury were localized predominantly in lysosome-like inclusions within the receptor cells, while those produced by HgCl{sub 2} exposure were situated mainly along the borders of neighboring cells. The present findings that organic and inorganic mercury compoundsmore » were deposited in the olfactory system along its whole length, from the receptor cell apices to the brain, support the electrophysiological results presented elsewhere.« less

Authors:
;  [1]
  1. Univ. of Aarhus, (Denmark)
Publication Date:
OSTI Identifier:
5735003
Resource Type:
Journal Article
Journal Name:
Ecotoxicology and Environmental Safety; (USA)
Additional Journal Information:
Journal Volume: 20:3; Journal ID: ISSN 0147-6513
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; MERCURY; TISSUE DISTRIBUTION; METHYLMERCURY; OLFACTORY BULBS; SENSITIVITY; SALMON; CONTAMINATION; MERCURY 203; METABOLISM; TRACER TECHNIQUES; ANADROMOUS FISHES; ANIMALS; AQUATIC ORGANISMS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ELEMENTS; EVEN-ODD NUCLEI; FISHES; HEAVY NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; MERCURY COMPOUNDS; MERCURY ISOTOPES; METALS; NERVOUS SYSTEM; NUCLEI; ORGANIC COMPOUNDS; ORGANIC MERCURY COMPOUNDS; ORGANS; RADIOISOTOPES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550501 - Metabolism- Tracer Techniques

Citation Formats

Baatrup, E, and Doving, K B. Histochemical demonstration of mercury in the olfactory system of salmon (Salmo salar L. ) following treatments with dietary methylmercuric chloride and dissolved mercuric chloride. United States: N. p., 1990. Web. doi:10.1016/0147-6513(90)90007-R.
Baatrup, E, & Doving, K B. Histochemical demonstration of mercury in the olfactory system of salmon (Salmo salar L. ) following treatments with dietary methylmercuric chloride and dissolved mercuric chloride. United States. https://doi.org/10.1016/0147-6513(90)90007-R
Baatrup, E, and Doving, K B. 1990. "Histochemical demonstration of mercury in the olfactory system of salmon (Salmo salar L. ) following treatments with dietary methylmercuric chloride and dissolved mercuric chloride". United States. https://doi.org/10.1016/0147-6513(90)90007-R.
@article{osti_5735003,
title = {Histochemical demonstration of mercury in the olfactory system of salmon (Salmo salar L. ) following treatments with dietary methylmercuric chloride and dissolved mercuric chloride},
author = {Baatrup, E and Doving, K B},
abstractNote = {The deposition of organic and inorganic mercury compounds was studied histochemically in the salmon (Salmo salar L.) olfactory system. One group of salmon was given fodder pellets containing methylmercuric chloride (CH{sub 3}HgCl, 99 micrograms Hg/g) for 4 weeks. Other groups of fish were exposed to dissolved mercuric chloride (HgCl{sub 2}, 270 micrograms Hg/liter) for 2, 6, and 12 hr, respectively. In both series of experiments, the radioisotope {sup 203}Hg was included in order to determine the accumulation of mercury in the olfactory system. Gamma-spectrometry showed that both mercury compounds accumulated in the olfactory rosettes and their nerves. Tissue sections from the rosettes and olfactory nerves were subjected to autometallographic silver enhancement, thereby rendering mercury deposits visible for light and electron microscopy. Microscopic analysis demonstrated an intense and comprehensive Hg deposition in the axons and Schwann cells of both methylmercury- and inorganic mercury-exposed fish. On the other hand, the two mercury compounds showed different staining patterns in the sensory epithelium. The silver grains evoked by methylmercury were localized predominantly in lysosome-like inclusions within the receptor cells, while those produced by HgCl{sub 2} exposure were situated mainly along the borders of neighboring cells. The present findings that organic and inorganic mercury compounds were deposited in the olfactory system along its whole length, from the receptor cell apices to the brain, support the electrophysiological results presented elsewhere.},
doi = {10.1016/0147-6513(90)90007-R},
url = {https://www.osti.gov/biblio/5735003}, journal = {Ecotoxicology and Environmental Safety; (USA)},
issn = {0147-6513},
number = ,
volume = 20:3,
place = {United States},
year = {Sat Dec 01 00:00:00 EST 1990},
month = {Sat Dec 01 00:00:00 EST 1990}
}