Androgen and FSH synergistically stimulate lipoprotein degradation and utilization by ovary granulosa cells

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Steroids; (United States)

DOI:https://doi.org/10.1016/0039-128X(84)90056-4· OSTI ID:5729590

Schreiber, J R; Nakamura, K; Schmit, V; Weinstein, D B

Androgen can directly modulate the induction of steroidogenic enzymes by FSH (follicle stimulating hormone) in ovary granulosa cells. In studies of its mechanism of action, the authors examined the androgen effect on granulosa cell interaction with lipoproteins, the physiologic source of cholesterol. After granulosa cells were cultured for 48 hours with and without androgen and/or FSH, the cells were incubated for 24 hours with /sup 125/I-lipoproteins (human high density lipoprotein (HDL), rat HDL, or human low density lipoprotein (LDL)). The media were then analyzed for lipoprotein protein coat degradation products (mainly /sup 125/I-monoiodotyrosine) and progestin (mainly 20 alpha-dihydroprogesterone (20 alpha-DHP)). In the absence of FSH and androgen, 2 X 10(5) granulosa cells degraded basal levels of all three lipoproteins, but produced no measurable 20 alpha-DHP. The addition of 10(-7) M androstenedione (A), testosterone (T), or 5 alpha-dihydrotestosterone (DHT) had no effect on lipoprotein protein degradation or 20 alpha-DHP production. FSH alone stimulated lipoprotein protein degradation by 50 to 300% while the addition of androgen synergistically augmented the FSH-stimulated 20 alpha-DHP production as well as protein coat degradation of all three lipoproteins. DHT and T were both effective, indicating that androgens themselves, and not estrogen products, were responsible for the effect on lipoprotein protein degradation and 20 alpha-DHP production.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 5729590
• Journal Information: Steroids; (United States), Vol. 43:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
BIOLOGICAL EFFECTS
LIPOPROTEINS
DECOMPOSITION
CELL CULTURES
CHOLESTEROL
FSH
IODINE 125
LABELLED COMPOUNDS
OVARIES
PROGESTERONE
SYNERGISM
TESTOSTERONE
TRACER TECHNIQUES
TYROSINE
AMINO ACIDS
ANDROSTANES
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
GONADOTROPINS
GONADS
HORMONES
HYDROXY ACIDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIPIDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
PROTEINS
RADIOISOTOPES
STEROID HORMONES
STEROIDS
STEROLS
550201* - Biochemistry- Tracer Techniques