Developmental differences in posttranslational calmodulin methylation in pea plants
- Univ. of Tennessee, Knoxville (USA)
A calmodulin-N-methyltransferase was used to analyze the degree of lysine-115 methylation of pea calmodulin. Calmodulin was isolated from segments of developing roots of young etiolated and green pea plants and was tested for its ability to be methylated by the calmodulin methyltransferase in the presence of {sup 3}H-methyl-S-adenosylmethionine. Calmodulin methylation levels were lower in apical root segments and in the young lateral roots compared with the mature, differentiated root tissues. The methylation of these calmodulin samples occurs specifically at lysine 115 since site-directed mutants of calmodulin with substitutions at this position were not methylated and competitively inhibited methylation. The present findings, combined with previous data showing differences in NAD kinase activation by methylated and unmethylated calmodulins, raise the possibility that posttranslational methylation could affect calmodulin action.
- OSTI ID:
- 5725802
- Report Number(s):
- CONF-9007196-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
Similar Records
N-methylation of calmodulin
Selective carboxyl methylation of structurally altered calmodulins in Xenopus oocytes
Related Subjects
CALMODULIN
METHYLATION
PHOSPHOTRANSFERASES
PISUM
PLANT GROWTH
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CHEMICAL REACTIONS
ENZYMES
GROWTH
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
PROTEINS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques