Occurrence and metabolism of 4-substituted glutamic acids in the seedlings of various species of legumes. [Sophora japonica]
The authors measured the levels of 4-methyleneglutamic acid (Meglu), 4-methyleneglutamine (Megln), erythro-4-methylglutamic acid (e-Mglu), and threo-4-methylglutamic acid (t-Mglu) in seedlings of various species of legumes by HPLC and ion exchange chromatography. High levels of e-Mglu and Megln but no t-Mglu or Meglu are present in Sophora japonica. Peanut seedling contain both e-Mglu and t-Mglu at 20-50% and 5%, resp., of the level of Meglu whereas only traces of Meglu and Mglu occur in soybean seedlings. Excised peanut embryos germinated on Linsmaier and Skoog medium + (U-/sup 14/C)-leucine incorporated isotope into e-Mglu, Meglu, and Megln; (U-/sup 14/C)-proline or glycine was not so incorporated. Soybean embryos rapidly converted added (2-/sup 14/C)-Meglu to a variety of non-amino acid products; peanut embryos, in contrast, retain 25% of added Meglu unchanged and 50% as Megln. These results suggest that in a variety of legumes leucine may serve as a precursor of Mglu and Meglu during germination; also, whereas Meglu remains as such or as Megln in some species, it is rapidly metabolized in others.
- Research Organization:
- Univ. of Michigan, Ann Arbor
- OSTI ID:
- 5725747
- Report Number(s):
- CONF-8707108-
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINO ACIDS
METABOLISM
SEEDLINGS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
GLUTAMIC ACID
GLYCINE
GLYCINE HISPIDA
ION EXCHANGE CHROMATOGRAPHY
LEGUMINOSAE
LEUCINE
LIQUID COLUMN CHROMATOGRAPHY
PROLINE
TRACER TECHNIQUES
TREES
AMINES
AZOLES
BACTERIA
CARBOXYLIC ACIDS
CHROMATOGRAPHY
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRROLES
PYRROLIDINES
RHIZOBIUM
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques