Soybean roots retain the seed urease isozyme synthesized during embryo development

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5724843

Torisky, R S; Polacco, J C ^[1]

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1. Univ. of Missouri, Columbia (USA)

Roots of young soybean plants contain two urease isozymes which are separable by hydroxyapatite chromatography. These two urease species (HAP1 and HAP2) differ in: (1) native gel electrophoretic mobility, (2) pH optima, and (3) recognition by a monoclonal antibody specific for the embryo-specific urease. By these parameters HAP1 is similar to the abundant embryo-specific urease isozyme while HAP2 resembles the ubiquitous urease, found in all soybean tissues previously examined (embryo, seed coat, cultured cells). Roots of mutant soybean plants lacking the seed urease contain no HAP1 urease activity, whereas roots of mutants lacking the ubiquitous urease contain no HAP2 urease activity. However, adventitious roots generated from cuttings of any urease genotype lack HAP1 urease activity. Furthermore, ({sup 35}S) methionine labelling shows no {und de novo} synthesis of the HAP1 urease in the root, and total root HAP1 urease activity decreases sharply following germination. We conclude: (1) HAP1 is a remnant of the seed urease accumulated in the embryonic root axis during seed development, and (2) HAP2 is ubiquitous urease synthesized de novo in the root.

• OSTI ID: 5724843
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
UREASE
METABOLISM
GLYCINE HISPIDA
ISOENZYMES
PLANT GROWTH
SULFUR 35
SULFUR COMPOUNDS
TRACER TECHNIQUES
AMIDINASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
GROWTH
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LEGUMINOSAE
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NON-PEPTIDE C-N HYDROLASES
NUCLEI
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
550501* - Metabolism- Tracer Techniques