Role of liver lysosomes in uptake and biliary excretion of manganese in mice
To determine the mechanism of manganese excretion from the liver into bile, manganese content in blood, bile, whole liver, and subcellular fractions of liver was measured following intraperitoneal injection of a single dose of manganese (49 mg Mn(OAc)/sub 2/. 4H/sub 2/O/kg body wt) in mice. Manganese in blood was rapidly incorporated into liver and appeared in bile within hours. Most of the absorbed manganese in liver was recovered in the mitochondrial-lysosomal fraction. Purified nuclei contained only one-tenth of the manganese found in the crude nuclear fraction. Sucrose density gradient centrifugation revealed that lysosomes took up and released manganese more rapidly than mitochondria. Also, manganese content in the liver and bile decreased following pretreatment with trypan blue. These values returned to normal with simultaneous administration of cortisone. Manganese released in vitro from the mitochondrial-lysosomal fraction and manganese in bile were measured as manganese acetate by gel filtration. The results suggest that liver lysosomes play an important role in intrahepatic movement and affect biliary excretion of absorbed manganese.
- Research Organization:
- Gunma Univ., Maebashi, Japan
- OSTI ID:
- 5724704
- Journal Information:
- Environ. Res.; (United States), Vol. 26:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MANGANESE
BIOLOGICAL EFFECTS
EXCRETION
UPTAKE
BILE
BLOOD
LIVER
LYSOSOMES
MICE
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CELL CONSTITUENTS
CLEARANCE
DIGESTIVE SYSTEM
ELEMENTS
GLANDS
MAMMALS
MATERIALS
METALS
ORGANOIDS
ORGANS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)