Limited proteolysis of human leukocyte interferon-. cap alpha. 2 and localization of the monoclonal antibody-binding antigenic determinant
Large peptide fragments of human leukocyte interferon-..cap alpha..2 (INF-..cap alpha..2) were produced by limited proteolysis with trypsin, pepsin, thermolysin, and Bacillus amyloliquefaciens serine proteinase, and the ability of the fragments to react with murine monoclonal antibodies NK2, directed toward INF-..cap alpha..2, was studied by the immunoblotting technique. The region of the sequence 110-149 is the most sensitive to proteinase attack and evidently is exposed on the surface of the INF-..cap alpha..2 molecule. The INF-..cap alpha..2 fragments 1-139, 1-147, and 1-149 react with antibodies, whereas the fragments 1-109 and 1-112 do not bind NK2 antibodies. A comparison of the primary structure of the families of human leukocyte and murine leukocyte INF in the region of the sequence 110-139 and an analysis of the ability of human INF differing in amino acid sequence to interact with NK2 antibodies suggested that the antigenic determinant that binds monoclonal antibodies NK2 is the sequence Glu/sub 114/-Asp/sub 115/-Ser/sub 116/-He/sub 117/ of the INF-..cap alpha..2 molecule.
- Research Organization:
- All-Union Scientific-Research Institute of the Genetics and Selection of Industrial Microorganisms, Moscow, USSR
- OSTI ID:
- 5722102
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 50:11; Other Information: Translated from Biokhimiya; 50: No. 11, 1859-1865(Nov 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INTERFERON
PROTEOLYSIS
RADIOIMMUNOASSAY
MONOCLONAL ANTIBODIES
RECEPTORS
PEPTIDES
BIOCHEMISTRY
AMINO ACID SEQUENCE
BINDING ENERGY
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYMES
LEUKOCYTES
MAN
PROTEINS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ENERGY
GROWTH FACTORS
HYDROLYSIS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LYMPHOKINES
LYSIS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PRIMATES
RADIOASSAY
RADIOIMMUNOLOGY
SOLVOLYSIS
TRACER TECHNIQUES
VERTEBRATES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques