A metal-ion NMR investigation of the antibiotic facilitated transport of monovalent cations through the walls of phospholipid vesicles. II. Sulfur-33 NMR

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5719011

Buster, D C

A technique has been developed to investigate the antibiotic facilitated transmembrane transport of monovalent cations using {sup 23}Na and {sup 7}Li Nuclear Magnetic Resonance spectroscopy. The initial portion of this thesis outlines the production and characterization of a model lipid system amenable to the NMR detection of cation transport. Large unilamellar vesicles (LUV) have been prepared from a 4:1 mixture of phosphatidylcholine and phosphatidylglycerol. The presence of the anionic chemical shift reagent dysprosium (III) tripolyphosphate, either inside or outside of the vesicles, allows for the spectroscopic separation of the NMR resonances arising from the inter- and extravesicular cation pools. The cation transporting properties of the channel-forming pentadecapeptide, gramicidin D, have been studied using the NMR technique.

• Research Organization: Arkansas Univ., Fayetteville, AR (USA)
• OSTI ID: 5719011
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBIOTICS
CHEMICAL PROPERTIES
CATIONS
MEMBRANE TRANSPORT
PHOSPHOLIPIDS
CHEMICAL COMPOSITION
CHEMICAL SHIFT
LITHIUM 7
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
REAGENTS
SODIUM 23
SULFUR 33
TRACER TECHNIQUES
ALKALI METAL ISOTOPES
ANTI-INFECTIVE AGENTS
CHARGED PARTICLES
DRUGS
ESTERS
EVEN-ODD NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LITHIUM ISOTOPES
MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
RESONANCE
SODIUM ISOTOPES
SPECTRA
STABLE ISOTOPES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques