Effect of iron chelators on placental uptake and transfer of iron in rat
The uptake of radiolabeled transferrin and iron by the rat placenta has been studied using two approaches. The first involved injection of a ferrous or ferric iron chelator followed by injection of label. Neither chelator decreased the amount of labelled transferrin in the placenta after 2-h incubation and only bipyridine, a ferrous iron chelator, inhibited iron transport to the fetus. Deferoxamine (DFO), a ferric iron chelator, had no effect on iron transport to the fetus but reduced iron uptake by the liver. Both bipyridine and DFO increased iron excretion into the gut and by the urinary tract to the same degree into the gut, but there was a 10-fold greater urinary excretion with bipyridine than with DFO. Injection of iron attached to the chelators showed that neither bipyridine nor DFO could donate iron to the fetus as efficiently as transferrin. The mechanism involved was further investigated by studying the effect of the chelators on uptake of transferrin-bound iron by placental cells in culture. DFO inhibited iron accumulation more effectively than bipyridine in the cultured cells. The effect was not due to a decrease in the cycling time of the receptor. The results can be explained if the iron is released from the transferrin in intracellular vesicles in the ferrous form, where it may be chelated by bipyridine and prevented from passing to the fetus or converted to the ferric form once it is inside the cell matrix.
- Research Organization:
- Univ. of Western Australia, Nedlands
- OSTI ID:
- 5716361
- Journal Information:
- Am. J. Physiol.; (United States), Vol. 252:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIPYRIDINES
BIOLOGICAL EFFECTS
DEFEROXAMINE
IRON 59
BLOOD-PLASMA CLEARANCE
UPTAKE
ALBUMINS
IN VITRO
IODINE 125
IODINE 131
IRON CHLORIDES
PLACENTA
RATS
TRANSFERRIN
AMINES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHELATING AGENTS
CHLORIDES
CHLORINE COMPOUNDS
CLEARANCE
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FETAL MEMBRANES
GLOBULINS
GLOBULINS-BETA
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
IRON COMPOUNDS
IRON ISOTOPES
ISOTOPES
MAMMALS
MEMBRANES
METALLOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIDINES
RADIOISOTOPES
RODENTS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques