The response of murine intestinal crypts to short-range promethium-147 beta irradiation: Deductions concerning clonogenic cell numbers and positions
An exteriorized loop of mouse intestine was exposed to /sup 147/Pm low-energy electrons, where the dose rate decreased by a factor of 5 from the base of the crypt to the top of the proliferative zone. A crypt survival curve was obtained, expressed in terms of exposure time. The shape of the curve was interpreted in terms of survival parameters for colony-forming cells (clonogens) derived using /sup 137/Cs gamma rays and the depth-dose curve measured for /sup 147/Pm electrons. It is concluded that the shape of the crypt survival curve using /sup 147/Pm electrons is inconsistent with the notion of either the presence of a large number of clonogens or a small number near the top of the proliferative zone. A computer fitting procedure showed that the best agreement between predicted and observed curves was achieved with 2.7 +/- 0.5 clonogens at cell position 5.6 +/- 0.6, in the putative stem-cell zone.
- Research Organization:
- Paterson Institute for Cancer Research, Manchester (England)
- OSTI ID:
- 5715312
- Journal Information:
- Radiat. Res.; (United States), Vol. 118:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SMALL INTESTINE
BIOLOGICAL RADIATION EFFECTS
BETA PARTICLES
CELL PROLIFERATION
COLONY FORMATION
DOSE-RESPONSE RELATIONSHIPS
ELECTRONS
MICE
PROMETHIUM 147
SURVIVAL CURVES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CHARGED PARTICLES
DIGESTIVE SYSTEM
ELEMENTARY PARTICLES
FERMIONS
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPES
LEPTONS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PROMETHIUM ISOTOPES
RADIATION EFFECTS
RADIOISOTOPES
RARE EARTH ISOTOPES
RARE EARTH NUCLEI
RODENTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals