Mouse. cap alpha. -globin genes and. cap alpha. -globin-like pseudogenes are not syntenic
- Oak Ridge National Lab., TN
A genetic polymorphism for a Bgl I endonuclease site near the ..cap alpha..-globin-like pseudogene ..cap alpha..-4 of C57BL/6 and C3H/HeN mice was used to show that ..cap alpha..-4 was not affected by three independent mutations in which the adult globin genes ..cap alpha..-1 and ..cap alpha..-2 were deleted. These results indicated that ..cap alpha..-4 might not be located adjacent to the adult ..cap alpha..-globin genes on chromosome 11. Restriction endonuclease analysis of DNA of a primary clone of a Chinese hamster-mouse somatic cell hybrid that had lost mouse chromosomes 11 and 18 showed that this clone lacked the adult murine globin genes ..cap alpha..-1 and ..cap alpha..-2 but it did contain the ..cap alpha..-globin-like pseudogenes ..cap alpha..-3 and ..cap alpha..-4. These results indicated that the adult ..cap alpha..-globin genes and ..cap alpha..-globin-like pseudogenes are not located on the same chromosome. Similar analyses of several other Chinese hamster-mouse somatic cell hybrids that had segregated other mouse chromosomes indicated that the ..cap alpha..-globin-like pseudogenes ..cap alpha..-3 and ..cap alpha..-4 are located on mouse chromosomes 15 and 17, respectively. These data explain why ..cap alpha..-3 and ..cap alpha..-4 were not affected by the three independently induced deletion-type mutations that cause ..cap alpha..-thalassemia in the mouse.
- OSTI ID:
- 5715013
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 78:10
- Country of Publication:
- United States
- Language:
- English
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CHROMOSOMES
DNA
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SOMATIC CELLS
ANIMAL CELLS
ANIMALS
BIOLOGY
CARBOXYLIC ACIDS
ENZYMES
ESTERASES
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
MAMMALS
NUCLEIC ACIDS
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ORGANIC COMPOUNDS
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550400* - Genetics