Inductively coupled plasma mass spectrometry applied to isotopic analysis of iron in human fecal matter
Inductively coupled plasma mass spectrometry combined with stable isotope dilution is applied to accurate isotopic analysis of human fecal matter for /sup 54/Fe and /sup 58/Fe. Argon plasma generated interferences are of minor concern. The interference from /sup 54/Cr can be corrected instrumentally, whereas /sup 58/Ni must be removed chemically. The ratio of the stable isotopes of interest can be measured routinely with a relative standard deviation of about 1%. The overall accuracy of the method for quantitative isotopic analyses is evaluated in Standard Reference Material (SRM) 1577a (Bovine Liver), fecal homogenate subsamples, and synthetic solutions of iron. For SRM 1577a, the respective comparisons are (..mu..g/g) 192.2 +/- 2.2 (present method) vs. 194 +/- 20 (certified value). For the fecal matrix, the present method yields (..mu..g/mL) 15.14 +/- 0.36 vs. 15.82 +/- 0.48 based on atomic absorption spectrophotometry. For an iron solution (250 ppm), replicate analyses yield the value of 245.4 +/- 1.5 ppm.
- Research Organization:
- Boston Univ. School of Medicine, MA
- OSTI ID:
- 5698086
- Journal Information:
- Anal. Chem.; (United States), Vol. 58:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
FECES
QUANTITATIVE CHEMICAL ANALYSIS
IRON 54
ISOTOPE DILUTION
MASS SPECTROSCOPY
IRON 58
ARGON
CHROMIUM 54
EXPERIMENTAL DATA
INTERFERING ELEMENTS
ISOTOPE RATIO
NICKEL 58
PLASMA
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
CHEMICAL ANALYSIS
CHROMIUM ISOTOPES
DATA
ELEMENTS
EVEN-EVEN NUCLEI
FLUIDS
GASES
INFORMATION
INTERMEDIATE MASS NUCLEI
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NICKEL ISOTOPES
NONMETALS
NUCLEI
NUMERICAL DATA
RARE GASES
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
WASTES
400104* - Spectral Procedures- (-1987)